CD11b/c (Microglia) MicroBeads have been developed for the magnetic isolation of rat cells based on the expression of the CD11b and CD11c antigens.

Data and images for CD11b/c (Microglia) MicroBeads, rat

Figures

Figure 1

CD11b/c
+
cells were isolated from day P3 postnatal rat brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS Dissociator, CD11b/c (Microglia) MicroBeads, a MiniMACS™ Separator, and two MS Columns. Cells were fluorescently stained with CD11b/c-FITC (# 130-105-273) in combination with CD45-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before MicroBead labeling
Before isolation
View details

Figure 1

CD11b/c
+
cells were isolated from day P3 postnatal rat brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS Dissociator, CD11b/c (Microglia) MicroBeads, a MiniMACS™ Separator, and two MS Columns. Cells were fluorescently stained with CD11b/c-FITC (# 130-105-273) in combination with CD45-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

CD11b/c
+
cells were isolated from day P3 postnatal rat brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS Dissociator, CD11b/c (Microglia) MicroBeads, a MiniMACS™ Separator, and two MS Columns. Cells were fluorescently stained with CD11b/c-FITC (# 130-105-273) in combination with CD45-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Negative fraction
Isolated CD11b/c
+
cells
View details

Figure 1

CD11b/c
+
cells were isolated from day P3 postnatal rat brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS Dissociator, CD11b/c (Microglia) MicroBeads, a MiniMACS™ Separator, and two MS Columns. Cells were fluorescently stained with CD11b/c-FITC (# 130-105-273) in combination with CD45-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

CD11b/c
+
cells were isolated from day P3 postnatal rat brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS Dissociator, CD11b/c (Microglia) MicroBeads, a MiniMACS™ Separator, and two MS Columns. Cells were fluorescently stained with CD11b/c-FITC (# 130-105-273) in combination with CD45-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD11b/c (Microglia) MicroBeads, rat

Overview

CD11b/c (Microglia) MicroBeads have been developed for the magnetic isolation of rat cells based on the expression of the CD11b and CD11c antigens.

Detailed product information

Background information

Background information
The CD11b/c (Microglia) antibody appears to recognize a common epitope shared between CD11b and CD11c. CD11b, also known as integrin alpha M or Mac-1, is a component of the complement factor 3 (CR3), whereas CD11c, also called as
integrin alpha X, is a component of complement receptor 4 (CR4).
CD11b and CD11c are expressed on micoglia, macrophages, monocytes, granulocytes, NK cells, and dendritic cells.
For optimal results, the Neural Tissue Dissociation Kit (P) (# 130‑092‑628) is required for the generation of a single-cell suspension.

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