Anti-PSA-NCAM MicroBead Kit, human and mouse

Name: Anti-PSA-NCAM MicroBead Kit, human and mouse
Availability: InStock

Anti-PSA-NCAM MicroBead Kit, human and mouse

The Anti-PSA-NCAM MicroBead kit has been designed for the isolation of PSA-NCAM

⁺

neural progenitors from differentiated ES or iPS cell cultures.

Product data and images for Anti-PSA-NCAM MicroBead Kit, human and mouse

Figures

Figure 1

PSA-NCAM

⁺

cells were isolated from human pluripotent stem cell-derived neuronal precursor cells using the Anti-PSA-NCAM MicroBead Kit, an LS Column, and a MiniMACS™ Separator. Cells were analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation

PSA-NCAM

⁺

cells

Figure 1

PSA-NCAM

⁺

cells were isolated from human pluripotent stem cell-derived neuronal precursor cells using the Anti-PSA-NCAM MicroBead Kit, an LS Column, and a MiniMACS™ Separator. Cells were analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

PSA-NCAM

⁺

cells were isolated from human pluripotent stem cell-derived neuronal precursor cells using the Anti-PSA-NCAM MicroBead Kit, an LS Column, and a MiniMACS™ Separator. Cells were analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Anti-PSA-NCAM MicroBead Kit, human and mouse

Overview

The Anti-PSA-NCAM MicroBead kit has been designed for the isolation of PSA-NCAM

⁺

neural progenitors from differentiated ES or iPS cell cultures.

Detailed product information

Background information

The Anti-PSA-NCAM antibody recognizes an epitope on polysialic acid (PSA) which, in vertebrates, is found linked to the extracellular domain of the neural cell adhesion molecule (NCAM, CD56).¹ PSA-NCAM, the highly polysialated form of NCAM, is predominantly expressed

in embryonic and neonatal neural tissue.² In vitro differentiation of human and mouse pluripotent stem cells under defined conditions revealed that neuronal progenitors, e.g. long-term self-renewing neuroepithelial-like stem cells, express the surface marker PSA-NCAM.³ Magnetically enriched, in vitro generated PSA-NCAM positive neural progenitors can be further differentiated into defined neuronal subtypes.

Columns

LS or autoMACS

^®

Columns.

References for Anti-PSA-NCAM MicroBead Kit, human and mouse

Publications

Barral, S. et al. (2013)
Efficient neuronal
in vitro
and
in vivo
differentiation after immunomagnetic purification of mESC derived neuronal precursors.
Stem Cell Res. 10(2): 133-46

Data and images

Data and images for Anti-PSA-NCAM MicroBead Kit, human and mouse

Figures

Figure 1

PSA-NCAM

⁺

cells were isolated from human pluripotent stem cell-derived neuronal precursor cells using the Anti-PSA-NCAM MicroBead Kit, an LS Column, and a MiniMACS™ Separator. Cells were analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation

PSA-NCAM

⁺

cells

Figure 1

PSA-NCAM

⁺

cells were isolated from human pluripotent stem cell-derived neuronal precursor cells using the Anti-PSA-NCAM MicroBead Kit, an LS Column, and a MiniMACS™ Separator. Cells were analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

PSA-NCAM

⁺

cells were isolated from human pluripotent stem cell-derived neuronal precursor cells using the Anti-PSA-NCAM MicroBead Kit, an LS Column, and a MiniMACS™ Separator. Cells were analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications

Specifications for Anti-PSA-NCAM MicroBead Kit, human and mouse

Overview

The Anti-PSA-NCAM MicroBead kit has been designed for the isolation of PSA-NCAM

⁺

neural progenitors from differentiated ES or iPS cell cultures.

Detailed product information

Background information

The Anti-PSA-NCAM antibody recognizes an epitope on polysialic acid (PSA) which, in vertebrates, is found linked to the extracellular domain of the neural cell adhesion molecule (NCAM, CD56).¹ PSA-NCAM, the highly polysialated form of NCAM, is predominantly expressed

in embryonic and neonatal neural tissue.² In vitro differentiation of human and mouse pluripotent stem cells under defined conditions revealed that neuronal progenitors, e.g. long-term self-renewing neuroepithelial-like stem cells, express the surface marker PSA-NCAM.³ Magnetically enriched, in vitro generated PSA-NCAM positive neural progenitors can be further differentiated into defined neuronal subtypes.

Columns

LS or autoMACS

^®

Columns.

References

References for Anti-PSA-NCAM MicroBead Kit, human and mouse

Publications

Barral, S. et al. (2013)
Efficient neuronal
in vitro
and
in vivo
differentiation after immunomagnetic purification of mESC derived neuronal precursors.
Stem Cell Res. 10(2): 133-46

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Anti-PSA-NCAM MicroBead Kit, human and mouse

Anti-PSA-NCAM MicroBead Kit, human and mouse

Product data and images for Anti-PSA-NCAM MicroBead Kit, human and mouse

Figures

Figure 1

Figure 1

Figure 1

Specifications for Anti-PSA-NCAM MicroBead Kit, human and mouse

Overview

Detailed product information

Background information

Columns

References for Anti-PSA-NCAM MicroBead Kit, human and mouse

Publications

Data and images

Data and images for Anti-PSA-NCAM MicroBead Kit, human and mouse

Figures

Figure 1

Figure 1

Figure 1

Specifications

Specifications for Anti-PSA-NCAM MicroBead Kit, human and mouse

Overview

Detailed product information

Background information

Columns

References

References for Anti-PSA-NCAM MicroBead Kit, human and mouse

Publications

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