MACS® Technology – Excellence and efficiency at your fingertips

MACS® Technology – Excellence and efficiency at your fingertips

Noteworthy references using MACS Technology


Flow cytometric analysis and sorting

Increased sensitivity and reduced run time in a flow cytometric sorter or analyzer with pre-enriched cells equals less stress on the cells. Magnetic cell separation will ensure reduced running cost for less cells and a higher quality of sorted cells.

Helios+ and Helios Treg subpopulations are phenotypically and functionally distinct and express dissimilar TCR repertoires. 
Thornton, A. et al. (2019), Eur. J. Immunol. 49:398-412.
Related technologies: Flow Cytometry

Checkpoint blockade accelerates a novel switch from an NKT-driven TNFα response toward  a T cell driven IFN-γ response within the tumor microenvironment 
Aoyama, S. et al. (2021), Journal for Immunotherapy of Cancer, 2021;9:e002269. 
Related technologies: Flow Cytometry 

Standardized 11-color flow cytometry panel for the functional phenotyping of human T regulatory cells.
Manuszak, C. et al. (2020), J. Biol. Methods, 7(2):e131.
Related technologies: Flow Cytometry

Immune checkpoint blockade to improve tumor infiltrating lymphocytes for adoptive cell therapy.
Kodumudi, K. et al. (2016), PLoS One, 11(4): e0153053. 
Related technologies: Flow Cytometry

CD11cHi monocyte-derived macrophages are a major cellular compartment infected by Mycobacterium tuberculosis.
Lee, J. et al. (2020), PLoS Pathogens, 16(6): e1008621.
Related technologies: Flow Cytometry

Modest Declines in Proteome Quality Impair Hematopoietic Stem Cell Self-Renewal.
Hidalgo San Jose, L. et al. (2020), Cell Reports, 30, 69–80.  
Related technologies: Flow Cytometry and sorting

SIRT1 is downregulated by autophagy in senescence and ageing. 
Xu, C. et al. (2020), Nature Cell Biology, 22, 1170–1179. 
Related technologies: Flow Cytometry

Combination Immune Checkpoint Blockade to Reverse HIV Latency.
Van der Sluis, R. (2020), J. Immunol, 204 (5) 1242-1254. 
Related technologies: Flow Cytometry 

Mouse models of neutropenia reveal progenitor-stage-specific defects.
Muench, D.E. et al. (2020), Nature, 582, 109–114. 
Related technologies: Flow Cytometry 

Thymic iNKT single cell analyses unmask the common developmental program of mouse innate T cells.
Harsha Krovi, S. et al. (2020), Nature Comm, 11:6238.
Related technologies: Flow Cytometry 


Workflow solutions for the preparation of cells prior to genomic analysis


Next Generation Sequencing (NGS)

For any application of NGS, the quality of the cells being sequenced is important. Standardization of the cell isolation process via automation with the autoMACS Pro Separator and MACS Technology ensures reproducibility, cell quality, and purity for NGS analysis.

Tumor cell enrichment by tissue suspension enables detection of mutations with low variant allele frequency and estimation of germline mutations
Hatakeyama, K. et al. (2022) Sci Rep 12: 2953
Related technologies: Whole exome sequencing (WES)

A metastasis map of human cancer cell lines. 
Jin, X. et al. (2020), Nature 588: 331–36. 
Related technologies: single-cell RNA sequencing (scRNA-seq)

Self-renewing resident cardiac macrophages limit adverse remodeling following myocardial infarction.
Dick, SA. et al. (2019),  Nat. Immunol. 20: 29–39. 
Related technologies: scRNA-seq

Single-cell transcriptomics of the human retinal pigment epithelium and choroid in health and macular degeneration. 
Voigt, AP. et al. (2019), PNAS. 116: 24100–107. 
Related technologies: scRNA-seq

Detection of neoantigen-reactive T cell clones based on the clonal expansion using next-generation sequencing of T cell receptor β complementarity-determining region 3.
Sumimoto, H. et al. (2020) JIM. 476: 112679. 
Related technologies: TCR sequencing (TCR-seq) 

TCR repertoire analysis reveals mobilization of novel CD8+ T cell clones into the cancer-immunity cycle following anti-CD4 antibody administration. 
Aoki, H. et al. (2018), Front. Immunol. 9: 3185.  
Related technologies: TCR-seq

The NASA twins study: A multidimensional analysis of a year-long human spaceflight.
Garrett-Bakelman, FE. et al. (2019), Science 364: 6436.
Related technologies: TCR-seq | DNA methylation assays  | Telomere length measurement 

Interferon-Λ3 exacerbates the inflammatory response to microbial ligands: Implications for SARS-CoV-2 pathogenesis. 
Read, SA. et al. (2021), J. Inflamm. Res. 14: 1257–70. 
Related technologies:  RNA-seq

Genetic characterization of a unique neuroendocrine transdifferentiation prostate circulating tumor cell-derived explant model. 
Faugeroux, VF. et al. (2020), Nat. Commun. 11: 1884.  
Related technologies: Whole exome sequencing (WES) | RNA-seq 

The use of PanDrugs to prioritize anticancer drug treatments in a case of T-ALL based on individual genomic data.
Fernández-Navarro, P. et al. (2019) T. BMC Cancer. 19: 1005. 
Related technologies: WES | RNA-seq

Next-generation sequencing (NGS) analysis on single circulating tumor cells (CTCs) with no need of whole-genome amplification (WGA).
Palmirotta, F. et al. (2017)  Cancer Genom. Proteom. 14: 173–79. 
Related technologies: Whole-genome sequencing (WGS)

Regulation of the methylome in differentiation from adult stem cells may underpin vitamin D risk in MS.
Ong, LTC. et al. (2020),  Genes Immun. 21: 335–47. 
Related technologies: Whole-genome bisulfite sequencing (WGBS)

The DNA methylation inhibitor zebularine controls CD4+ T cell mediated intraocular inflammation. 
Zou, Y. et al. (2019), Front. Immunol. 10. 
Related technologies: WGBS | RNA-seq

Redox-dependent BMI1 activity drives in vivo adult cardiac progenitor cell differentiation.
Herrero, D. et al. (2018)  Cell Death Differ. 25: 809–22. 
Related technologies: ChIP sequencing (ChIP-seq)

Impaired microRNA processing in neutrophils from rheumatoid arthritis patients confers their pathogenic profile modulation by biological therapies.
Rosa, IA. et al. (2020),  Haematologica 105: 2250–61.
Related technologies: Nanostring nCounter

TNF-α–induced protein 3 is a key player in childhood asthma development and environment-mediated protection.
Krusche, J. et al. (2019),  J. Allergy Clin. Immunol. 144: 1684-1696.e12.
Related technologies: Nanostring nCounter

Asymptomatic infection of Marburg virus reservoir bats is explained by a strategy of immunoprotective disease tolerance. 
Guito, JC. et al. (2021), Curr. Biol. 31: 257-270.e5.
Related technologies: Nanostring nCounter

Workflow solutions for the preparation of cells prior to genomic analysis

Find more information on full Miltenyi Biotec workflow solutions for the preparation of cells prior to genomic analysis.


CRISPR/Cas9 genome editing

Reliability and speed are important factors in getting published CRISPR/ Cas9 genome editing results. Isolation of target cells via MACS Technology increases the frequency of rare cells, and ensures a standardized process with high quality for optimal genome editing efficiency.

Ex vivo editing of human hematopoietic stem cells for erythroid expression of therapeutic proteins.
Pavani, G. et al. (2020), Nat. Commun. 11: 3778.  
Cell type: CD34+ cells  

Correction of β-thalassemia by CRISPR/Cas9 editing of the α-globin locus in human hematopoietic stem cells.
Pavani, G. et al. (2021), Blood Adv. 5: 1137–53.
Cell type: CD34+ cells

Large DNA methylation nadirs anchor chromatin loops maintaining hematopoietic stem cell identity. 
Zhang, X. et al. (2020), Mol. Cell. 78: 506-521.e6. 
Cell type: CD34+ cells

Activated invariant natural killer T cells directly recognize leukemia cells in a CD1d‐independent manner.
Aoki, T. et al. (2020), Cancer Sc. 7: 2223–33.
Cell type: iNKT cells

Kidney GATA3 + regulatory T cells play roles in the convalescence stage after antibody-mediated renal injury.
Sakai, R. et al. (2020), Cell. Mol. Immunol. 11: 1–13.
Cell type: CD4+ T cells

TraFo-CRISPR: Enhanced genome engineering by transient foamy virus vector-mediated delivery of CRISPR/Cas9 components. 
Lindel, F. et al. (2019),  Mol. Ther. Nucleic Acids. 18: 708–26.
Cell type: CD4+ T cells


Mass cytometry (CyTOF)

For CyTOF, pure samples offer a clean path to necessary markers and lead to improved results and analysis. Magnetic cell separation can optimize the quality of samples for mass cytometry.

Distinct immune cell populations define response to Anti-PD-1 monotherapy and Anti-PD-1/Anti-CTLA-4 combined therapy. 
Gide, TN. et al. (2019), Cancer Cell 35: 238-255.e6.  
Cell type: CD45+ cells 

Multiplex peptide-MHC tetramer staining using mass cytometry for deep analysis of the influenza-specific T cell response in mice.
Fehlings, M. et al. (2018), J. Immunol. Methods. 453: 30–36.  
Cell type: CD45+ cells 

Respiratory syncytial virus infects regulatory B cells in human neonates via chemokine receptor CX3CR1 and promotes lung disease severity. 
Zhivaki, D. et al. (2017), Immunity 46: 301–14.  
Cell type: CD19+ cells 

In-depth phenotypic characterization of reticulocyte maturation using mass cytometry.
Thomson-Luque, R. et al. (2018), Blood Cells Mol. Dis. 72: 22–33.  
Cell type: Red blood cells (RBC) 

Human skin is protected by four functionally and phenotypically discrete populations of resident and recirculating memory T cells.
Watanabe, R. et al. (2015), Sci. Transl. Med. 7: 279ra39-279ra39.  
Cell type: T cells 


Engineered immune cells

Automated magnetic cell separation results in a well-defined, enriched cell population ready for your CAR cell research. Together the autoMACS Pro and MACS Technology are key to obtain robust, standardized, and reproducible results.

Universal ready-to-use immunotherapeutic approach for the treatment of cancer: Expanded and activated polyclonal Γδ memory T cells.
Polito, VA. et al. (2019), Front. Immunol. 10 (2019). 
Cell type: γδ-T Cells GMP-Grade 

Vectofusin-1 improves transduction of primary human cells with diverse retroviral and lentiviral pseudotypes, enabling robust, automated closed-system manufacturing.
Radek, C. et al. (2019),  Hum. Gene Ther. 30: 1477–93.  
Related technologies: Induction of CAR-T 

CAR T cells releasing IL-18 convert to T-Bethigh FoxO1low effectors that exhibit augmented activity against advanced solid tumors.
Chmielewski, M. et al. (2017)  Cell Rep. 21: 3205–19.  
Cell type: CD4+ and CD8+ T cells 

Optimization of IL13Rα2-targeted chimeric antigen receptor T cells for improved anti-tumor efficacy against glioblastoma.
Brown, CE. et al. (2018), Mol. Ther. 26: 31–44.  
Cell type: CD45RO+ CD62L+ Tcm

Workflow solutions for CAR T cell research

Find more information on full workflow solutions for CAR T cell research.


Cellular Reprogramming (iPSC)

Reprogramming of somatic cells to an ESC-like state is an attractive approach to develop SC-based applications. Magnetic cell separation can enrich rare cell populations for iPSC generation and purify populations to ensure high viability and high reprogramming rate.

TET2 haploinsufficiency alters reprogramming into induced pluripotent stem cells.
Secardint, L. et al. (2020), Stem Cell Res. 44: 101755.  
Related technologies: iPSC generation from CD34+ cells

Optimised generation of IPSC-derived macrophages and dendritic cells that are functionally and transcriptionally similar to their primary counterparts. 
Monkley, S. et al. (2020), PLOS ONE 15: e0243807.  
Related technologies: iPSC-derived CD14+ cells

Disrupted filamin A/ΑIIbβ3 interaction induces macrothrombocytopenia by increasing RhoA activity. 
Donada, A. et al. (2019), Blood 133: 1778–88.  
Related technologies: iPSC generation from CD34+ cells 

NOX4 is the main NADPH oxidase involved in the early stages of hematopoietic differentiation from human induced pluripotent stem cells.
Brault, J. et al. (2020), Free Radic. Biol. Med. 146: 107–18.  
Related technologies: iPSC-derived CD34+ cells


Functional in vitro assays

Obtaining enriched cell populations with high viability and functional activity is key to in vitro assay success. Magnetic cell sorting ensures highly viable and pure cell populations are used in further functional assays.

Apoptotic tumor cell-derived microRNA-375 uses CD36 to alter the tumor-associated macrophage phenotype. 
Frank, AC. et al. (2019), Nat. Commun. 10: 1135.  
Related technologies: Spheroid tumor - immune cell coculture | Microscopy 

Astrocytic trans-differentiation completes a multicellular paracrine feedback loop required for medulloblastoma tumor growth. 
Yao, M. et al. (2020), Cell 180: 502-520.e19.  
Related technologies: Tumor - immune cell coculture | RNA-seq | Flow cytometry 

CD24 signaling through macrophage siglec-10 is a target for cancer immunotherapy.
Barkal, AA. et al. (2019), C Nature 572: 392–96.  
Related technologies: Phagocytosis assays | Time-lapse live-cell-microscopy 

Neoadjuvant PD-1 immune checkpoint blockade reverses functional immunodominance among tumor antigen-specific T cells. 
Friedman, J.  et al. (2020), Clin. Cancer Res. 26: 679–89.  
Related technologies: TIL culture | Cytokine production assays | Flow cytometry 

Inhibition of T helper cell differentiation by tacrolimus or sirolimus results in reduced B cell activation: Effects on T follicular helper cells. 
Kraaijeveld, R. et al. (2019), Transplant. Proc. 51: 3463–73. 
Related technologies: In vitro stimulation/co-culture | Flow cytometry

Pseudomonas aeruginosa infection impairs NKG2D-dependent NK cell cytotoxicity through regulatory T cell activation. 
Vourc’h, M. et al. (2020), Infect. Immun. 88: e00363-20 
Related technologies: Cell culture and infection of cells | RT-PCR | ELISA | Flow cytometry 



Proteomics & Metabolomics

Magnetic cell isolation of specific populations allows the study of proteome and metabolome in specific cell types with minimal to no alterations.

Systematic discovery and validation of T cell targets directed against oncogenic KRAS mutations
Choi, J. et al. (2021), Cell Reports Methods 1, 100084
Related technologies: Mass spectrometry | Sequencing

Persistence of adoptively transferred T cells with a kinetically engineered IL-2 receptor agonist. 
Parisi, G. et al. (2020), Nat. Commun. 11: 660.  
Related technologies: Single cell secretome via Isoplexis 

Targeted co-delivery of tumor antigen and α-galactosylceramide to CD141+ dendritic cells induces a potent tumor antigen-specific human CD8+ T cell response in human immune system mice. 
Huang, J. et al. (2020), Front. Immunol 11. 
Related technologies: Single cell secretome via Isoplexis 

Multi-omics resolves a sharp disease-state shift between mild and moderate COVID-19. 
Su, Y. et al. (2020), Cell 183: 1479-1495.e20. 
Related technologies: Single cell secretome via Isoplexis 

Population-specific metabolic alterations in professional antigen-presenting cells contribute to sepsis-associated immunosuppression.
Schenz, J. et al. (2020), Shock 53: 5–15. 
Related technologies: Seahorse 

Analyzing the impact of mycobacterium tuberculosis infection on primary human macrophages by combined exploratory and targeted metabolomics. 
Vrieling, F. et al. (2020), Sci. Rep. 10: 7085.  
Related technologies: Seahorse 

Adiponectin limits IFN-γ and IL-17 producing CD4 T cells in obesity by restraining cell intrinsic glycolysis. 
Surendar, J.  et al. (2019), Front. Immunol. 10.  
Related technologies: Seahorse 

Distinct regulation of Th17 and Th1 cell differentiation by glutaminase-dependent metabolism. 
Johnson, MO. et al. (2018), Cell 175: 1780-1795.e19. 
Related technologies: Seahorse | LC-High-Resolution MS analysis 

Fatty acid and carnitine metabolism are dysregulated in systemic sclerosis patients.
Ottria, A. et al. (2020) Front. Immunol. 11. 
Related technologies: LC-MS analysis 


In vivo applications

For efficient in vivo applications, the quality of cells being injected is important for successful engraftment. Highly pure and viable cell populations can be best obtained using MACS Technology.

Human Monocyte-Derived Suppressor Cell Supernatant Induces Immunoregulatory Effects and Mitigates xenoGvHD 
Gérard, C. et al. (2022) Front. Immunol. 10: 3389 
Related technologies: Flow Cytometry

Testing cancer immunotherapy in a human immune system mouse model: correlating treatment responses to human chimerism, therapeutic variables and immune cell phenotypes.
Marín-Jiménez, JA. et al. (2021) Front. Immunol. 12: 607282. 
Related technologies: Humanized mouse model

Bioimaging of alloantigen-stimulated regulatory T cells in rat vascularized composite allotransplantation.
Cheng, H-Y. et al. (2018), PLoS One, 13(9): e0203624. 
Related technologies: Allo-transplantation 

Genetic in vivo engineering of human T lymphocytes in mouse models. 
Weidner, T. et al. (2021), Nat. Protoc. 16: 3210–3240.  
Related technologies: Humanized mouse model 

Peripheral host T cells survive hematopoietic stem cell transplantation and promote graft-versus-host disease. 
Divito, SJ. et al. (2020), JCI. 130: 4624–36.  
Related technologies: Humanized mouse model 

Anti-tumor effects of anti-PD-1 antibody, pembrolizumab, in humanized NSG PDX mice xenografted with dedifferentiated liposarcoma.
Choi, B. et al. (2020),  Cancer Lett. 478: 56–69.  
Related technologies: Humanized mouse model 

Development of an adrenocortical cancer humanized mouse model to characterize anti-PD1 effects on tumor microenvironment. 
Lang, J. et al. (2020), JCEM 105: 26–42.  
Related technologies: Humanized mouse model

Interferon gamma mediates hematopoietic stem cell activation and niche relocalization through BST2
Florez, MA. et al. (2020), Cell Rep. 33: 108530.  
Related technologies: Stem cell homing studies 

Persistence of self-reactive CD8+ T cells in the CNS requires TOX-dependent chromatin remodeling.
Page, N. et al. (2021), Nat. Commun. 12: 1009. 
Related technologies: Adoptive T cell transfer 

Crucial role of CD69 in anti-tumor immunity through regulating the exhaustion of tumor-infiltrating T cells. 
Mita, Y. et al. (2018), Int. Immunol. 30: 559–67.  
Related technologies: Adoptive T cell transfer 

Pathogenic TH17 inflammation is sustained in the lungs by conventional dendritic cells and toll-like receptor 4 signaling.
Shalaby, KH. et al. (2018), J. Allergy Clin. Immunol. 142: 1229-1242.e6.  
Related technologies: Adoptive T cell transfer 

Persistence of self-reactive CD8+ T cells in the CNS requires TOX-dependent chromatin remodeling
Page, N. et al. (2021), Nat. Commun. 12: 1009.  
Related technologies: Adoptive T cell transfer 

Adoptive transfer of basophils enriched from mouse spleen.
Piliponsky, AM. et al. (2019), Bio Protoc. 9. 
Related technologies: Adoptive B cell transfer

Automated cell isolation at the push of a button

Publishable and reliable results can be yours as well with the ever-dependable autoMACS® Pro Separator. Investigate the instrument for yourself. 

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