Integrated cell activation, expansion, and polarization assays for evaluating immune responses

In response to certain activation stimuli, T and B lymphocytes, macrophages, NK, and dendritic cells undergo rapid proliferation, which is critical to initiate the immune response. Evaluating the dynamic changes, the cell interactions, and the secretion of cytokines in these highly-regulated initial stages of the immune response is crucial to understand and characterize the immunomodulatory efficacy and potency of novel therapeutic drugs. Cell activation, proliferation, or expansion assays are essential to these evaluations and determining potential immune responses.

The responsiveness of primary human T cells to therapeutic antibodies, immune checkpoint modulators, anti-PD-1 and anti-PD-L1, was assessed in an in vitro mixed-lymphocyte reaction. 

In this study, the receptivity of primary human T cells to therapeutic antibodies, immune checkpoint modulators, anti-PD-1 and anti-PD-L1, was assessed in an in vitro mixed-lymphocyte reaction. The investigated therapeutic antibodies demonstrated  a clear response for IFN-γ production.

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Mixed leukocyte reaction and other antigen-presentation assays

Learn all about Miltenyi Biotec’s antigen presentation assay workflows and solutions. Reliable tools covering each and every step of your workflow. From enrichment to differentiation and analysis.

Multicolor immunophenotyping results of invitro-generated macrophages characterized by expression of CD163, CD209, CD86, and CD200R

In this study by Janss et al., from ImmunXperts, a  suppression assay was performed with a co-culture of macrophages, CD4 T cells, and test products. Multicolor immunophenotyping demonstrated successful polarization and the overall success of the suppression assay for drug efficacy evaluation.

Webinar
Understanding how in vitro macrophage assays drive IO drug development forward

TAMs and the TME: Learn how pre-clinical in vitro techniques help predict the effect of immunotherapeutics in vivo. 

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