Microscope | |
Instrument type | Widefield epifluorescence microscope |
Camera | Equipped with a latest-generation sCMOS camera with high resolution and thermoelectrically cooled sensor(15 megapixel, large 25 mm diagonal field of view) |
Excitation | Six high-power excitation LEDs (filters: 386/23 nm, 420/10 nm, 470/40 nm, 531/46 nm, 628/32 nm, 725/40 nm) |
Emission | Five emission filters (470/40 nm, 530/43 nm, 580/25 nm, 698/70 nm, 809/81 nm) |
Autofocus | Dual approach of hardware- and image-based autofocus mechanisms |
Objectives | • 2× objective to generate overview images; NA 0.1 • 20× long working distance objective (designed for 1 mm thick slides); NA 0.45 • 20× objective with high numerical aperture (designed for 170 μm thick cover glass); NA 0.75 |
Bleaching unit | • Separate bleaching unit for optimized signal erasure • Illuminated area: 3 mm × 3 mm • Light intensity: 2 W |
Liquid handling system | |
Needle arm | Robotic needle arm allowing fully automated liquid transfer |
Steel needle | Washable stainless steel needle with minimized spillover |
Syringe pump | Syringe pump drive for accurate volumetric staining of samples |
Fluid containers | 1.5 L fluid containers for automatic operation over several days without the need to exchange containers |
Sample and reagent stage | |
Automated stage | Stage with sub-micron positioning accuracy of 100 nm (automated image registration for different cycles) |
Automated sample and reagent carrier positioning | Convenient loading and clamping of all supported sample carriers and reagents |
Technical data | |
Computer | Two integrated computers for experiment planning/execution and image analysis |
Monitors | • Tiltable integrated touch display (Full HD) for PC1 • External 4K monitor for PC2 |
Storage/ports | • 25 TB integrated storage to store data of several experiments • 2× USB 3.0 • HDMI port |
Network | 1× 10 GbE 1× RJ45 GbE/10GbE |
Power requirement and consumption | 100–240 V, 50/60 Hz, max. 7/13A, max. 1,300 W |
MACS iQ View Software | |
Control software | • Comprehensive sample and reagent management system • Easy planning and design of both simple and complex experiments • User-friendly execution and monitoring of experiments |
Analysis software | • Easy display of hundreds of markers from high-dimensional datasets • Fast and flexible segmentation • Interactive gating • Unbiased data analysis (k-means clustering, UMAP and t-SNE calculation) • Multiple plotting options (histogram, scatter plot, strip and violin plots, heatmaps) • Workflow editor • Distance analyses |
Size and weight | |
Dimensions | • Instrument dimension: 1,220 mm × 780 mm × 650 mm (w × d × h); footprint: 1,210 mm × 750 mm (w × d) • MACSima Table1: 1,250 mm × 765 mm × 813 mm (w × d × h) |
Weight | • Instrument: 160 kg • MACSima Table1: 120 kg |
1 The MACSima Imaging System is delivered with a trolley table specifically designed to support the system.
The MACS® iQ View Analysis Software was specifically developed to analyze the ultra high amount of data obtained in ultra high content imaging experiments by the MACSima Imaging System. Here are some of its key features:
The easy way to ultra high content imaging.
Order no. 130-121-164
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