CD34 antibodies, human

CD34 antibodies, human

Clone: AC136
The monoclonal antibody clone AC136 detects a class III epitope of the CD34 antigen. This epitope is different than the one recognized by the clone used in the CD34 MicroBead Kits.
The CD34 antigen is a single chain transmembrane glycoprotein, expressed on human hematopoitetic stem and progenitor cells, endothelial progenitor cells, vascular endothelial cells, embryonic fibroblasts, and some cells in fetal and adult nervous tissue. The antigen is absent on fully differentiated hematopoietic cells such as normal peripheral blood lymphocytes, monocytes, granulocytes, erythrocytes, and platelets. Clone AC136 has a similar specifity as the CD34 monoclonal antibody clone 8G12 (HPCA-2).
CD34 antibodies can be used for studies of hematopoiesis and nonhematopoiectic stem cells, phenotyping of hematopoietic stem cells, and studies on phenotyping of hematologic malignancies, endothelial cells, and endothelial progenitor cells (EPCs).

Alternative names

gp105-120, Mucosialin, My10

Technical specifications

  • Antigen: CD34
  • Clone: AC136
  • Isotype: mouse IgG2a
  • Isotype control: Mouse IgG2a – isotype control antibodies
  • Alternative names of antigen: gp105-120, Mucosialin, My10
  • Entrez Gene ID: 947
  • Molecular mass of antigen [kDa]: 37
  • Distribution of antigen: bone marrow, cancer stem cells, CNS cells, dendritic cells, endothelial cells, fibroblasts, hematopoietic stem cells, liver, mast cells, osteoblasts
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates:
  • Selected references

    1. Stull, R. A. et al. (2000) Simultaneous flow cytometric analyses of enhanced green and yellow fluorescent Cytometry 40: 126-134
    2. Metsuyanim, S. et al. (2009) Expression of stem cell markers in the human fetal kidney. PLoS One 4(68): e6709
    3. De Paepe, M. E. et al. (2011) Alveolar epithelial cell therapy with human cord blood-derived hematopoietic progenitor cells. Am. J. Pathol. 178(3): 1329-1339
    4. Bility, M. T. et al. (2012) Generation of a humanized mouse model with both human immune system and liver cells to model hepatitis C virus infection and liver immunopathogenesis. Nat. Protoc. 7(9): 1608-1617
    5. Vets, S. et al. (2012) Lens epithelium-derived growth factor/p75 qualifies as a target for HIV gene therapy in the NSG mouse model. Mol. Ther. 20(5): 908-917
    6. Lian, W. et al. (2014)
      Varying levels of 6-keto-prostaglandin F1α and thromboxane B2 in serum and endothelialization and hyperplasia in small-diameter grafts seeded with CD34
      +
      bone marrow cells in canines.
      Exp Ther Med. 7(5): 1123-1129
Product options: 1
130-108-040

CD34 pure, human

100 µg in 100 µL
DKK 1.230,00