Find the products and resources you are looking for!
Miltenyi Biotec distribution:
Human peripheral blood mononuclear cells (PBMCs) were stained with CD34 antibodies as well as with CD45 and CD15 antibodies. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. The cells were analyzed by flow cytometry using the MACSQuant®
Analyzer. A pre-gate of CD45+
cells was used. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandems.
Copyright © 2020 Miltenyi Biotec and/or its affiliates. All rights reserved.