Human IL-6

Human IL-6

IL-6 stands for interleukin 6. Human IL-6 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-6 can be used for a variety of applications, including:
  • Induction of colony formation from hematopoietic progenitor cells in semi-solid medium.
  • Replacement of feeder cells in the preparation of murine and human hybridomas.
  • In vitro differentiation of TH 17 cells.

Background information

IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine, which regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions. It induces, for instance, the terminal maturation of activated B cells into antibody-secreting plasma cells and acts in synergy with IL-3 to support the proliferation of hematopoietic stem cells. IL-6 is produced by many cell types, such as monocytes, fibroblasts, endothelial cells, T cells, etc. Disturbed IL-6 production has been associated with pathological processes including inflammatory autoimmune diseases, such as rheumatoid arthritis and cancer.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of B9 hybridoma cells (NIBSC 89/548)
  • premium grade: ≥ 5×
    10
    7
    IU/mg
    (typical activity: ≥ 1.3×
    10
    8
    IU/mg
    )
  • research grade: ≥ 2×
    10
    7
    IU/mg
  • Selected references

    1. Kaebisch R. et al. (2014) Helicobacter pylori cytotoxin-associated gene A impairs human dendritic cell maturation and function through IL-10-mediated activation of STAT3. J. Immunol. 192: 316-323
    2. Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
    3. Ghirelli, C. et al. (2010) Systematic cytokine receptor profiling reveals GM-CSF as a novel TLR-independent activator of human plasmacytoid predendritic cells. Blood 115(24): 5037-5040
    4. Gaines-Das, R.E. and Poole, S. (1993) The international standard for interleukin-6. Evaluation in an international collaborative study. J. Immunol. Methods 160: 147-153
    5. Dighe N. et al. (2014) Long-term reproducible expression in human fetal liver hematopoietic stem cells with a UCOE-based lentiviral vector. PLoS One 9(8): e104805
    6. Wright, C. et al. (2012) Detection of multiple autoantibodies in patients with ankylosing spondylitis using nucleic acid programmable protein arrays. Mol. Cell. Proteomics 11(2): M9.00384
    7. Brault, J. et al. (2014)
      Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
      ex vivo
      models of X
      0
      -linked, AR22
      0
      - and AR47
      0
      - chronic granulomatous diseases.
      Biores Open Access 3(6): 311-326
    8. Steinleitner, K. et al. (2012) EVI1 and MDS1/EVI1 expression during primary human hematopoietic progenitor cell differentiation into various myeloid lineages. Anticancer Res. 32(11): 4883-4889
Product options: 7
E. coli
10 µg
DKK 1.040,00 
E. coli
25 µg
DKK 1.615,00 
E. coli
10 µg
DKK 1.270,00 
E. coli
25 µg
DKK 2.040,00 
E. coli
100 µg
DKK 4.045,00 
E. coli
500 µg
DKK 10.550,00 
E. coli
1000 µg
DKK 17.095,00 

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