Human Flt3-Ligand

Human Flt3-Ligand

Flt3-Ligand stands for Fms-related tyrosine kinase 3 ligand. Human Flt3-Ligand is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human Flt3-Ligand can be used for a variety of applications, including:
  • In vitro expansion of CD34+ hematopoietic progenitor cells.
  • Differentiation of ES-derived cells towards the hematopoietic lineage.
  • In vitro generation of Langerhans cells, dendritic cells, or eosinophils from CD34+ cells.

Background information

Fms-related tyrosin kinase 3 ligand (Flt3-Ligand) is a growth factor that regulates early hematopoiesis. Flt3-Ligand belongs to a small family of α-helical cytokines and promotes in synergy with other growth factors like G-CSF, GM-CSF, SCF, and IL-3 the proliferation and differentiation of primitive hematopoietic stem cells. Early B cell lineage differentiation as well as expansion of monocytes and immature dendritic cells is stimulated. Flt3-Ligand is expressed by T lymphocytes and bone marrow stromal fibroblasts as a membrane-bound and a soluble isoform. Both isoforms signal through the tyrosine kinase receptor Flt3/Flk-2, which is restricted to cells of hematopoietic origin. Human Flt3-Ligand is also active on mouse cells.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of OCI-AML5 cells (NIBSC 96/532)
  • premium grade: ≥ 5×
    10
    5
    U/mg
  • research grade: ≥ 2×
    10
    5
    U/mg
  • Selected references

    1. Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
    2. Narla, A. et al. (2011) Dexamethasone and lenalidomide have distinct functional effects on erythropoiesis. Blood 118(8): 2296-2304
    3. Velardi, E. et al. (2014) Sex steroid blockade enhances thymopoiesis by modulating Notch signaling. J. Exp. Med. 211(12): 2341-2349
    4. Dussiau C. et al. (2015) Targeting IRAK1 in T-cell acute lymphoblastic leukemia. Oncotarget 6: 18956-18965
    5. Meyer, C. and Drexler, H.G. (1999) FLT3 ligand inhibits apoptosis and promotes survival of myeloid leukemia cell lines. Leuk. Lymphoma 32: 577-581
    6. Brault, J. et al. (2014)
      Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
      ex vivo
      models of X
      0
      -linked, AR22
      0
      - and AR47
      0
      - chronic granulomatous diseases.
      Biores Open Access 3(6): 311-326
Product options: 6
130-093-854

Human Flt3-Ligand, research grade

E. coli
10 µg
DKK 1.195,00 
130-096-474

Human Flt3-Ligand, research grade

E. coli
25 µg
DKK 2.235,00 
130-096-476

Human Flt3-Ligand, premium grade

E. coli
10 µg
DKK 1.465,00 
130-096-477

Human Flt3-Ligand, premium grade

E. coli
25 µg
DKK 2.810,00 
130-096-479

Human Flt3-Ligand, premium grade

E. coli
100 µg
DKK 4.850,00 
130-096-480

Human Flt3-Ligand, premium grade

E. coli
1000 µg
DKK 21.175,00