CD197 antibodies

CD197 antibodies

Clone: REA108
REA108 recognizes human CD197 which is a chemokine receptor with a C-C motif that mediates homing of T cells to secondary lymphoid organs via high endothelial venules (HEV) and is also known as CCR7. For example, naive T cells migrate very efficiently through lymph nodes using the adhesion molecules L-selectin, CD62L, and CCR7. Ligands for CCR7, e.g. CCL19, are expressed by the HEV of secondary lymphoid organs, by parenchymal cells within T cell zones of lymph nodes, and by endothelial cells at the openings of lymphatic vessels within peripheral tissues. Expression of CCR7 and the CD45RA isoform distinguishes three subsets of T cells: naive T cells (CCR7
+
CD45RA
+
), central memory T cells (CCR7
+
CD45RA
), and effector memory T cells (CCR7
CD45RA
).
Additional information: Clone REA108 displays negligible binding to Fc receptors.

Applications

Reagent can be used for immunophenotyping by flow cytometry. Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. It is important to understand the normal expression pattern for this antigen and its relationship to expression of other relevant antigens in order to perform appropriate analysis.
Expression of CD197 may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Alternative names

CCR7, BLR-2, CC-CKR-7, CCR-7, Cdw197, CMKBR7, EBI-1

Technical specifications

  • Antigen: CD197
  • Clone: REA108
  • Isotype: recombinant human IgG1
  • Alternative names of antigen: CCR7, BLR-2, CC-CKR-7, CCR-7, Cdw197, CMKBR7, EBI-1
  • Molecular mass of antigen [kDa]: 40
  • Distribution of antigen: B cells, dendritic cells, macrophages, NK cells, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates:
  • Selected references

    1. Bacher, P. et al. (2014)
      Identification of immunogenic antigens from
      Aspergillus fumigatus
      by direct multi parameter characterization of specific conventional and regulatory CD4
      +
      T cells.
      J. Immunol. 193: 3332-3343
    2. Sallusto, F. et al. (1999) Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Nature 401: 708-712
    3. Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
    4. Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
    5. van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
    6. Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
    7. Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of immunologically defined cell populations by flow cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition
  • Brochures and posters

  • Certificates

    Please follow this
    link
    to download the Certificate of Conformity (CoC) by lot number.
Product options: 1
170-078-041

CD197-PE

for 50
tests
DKK 2.540,00