CD10 antibodies

CD10 antibodies

Clone: 97C5
97C5 recognizes human CD10, the human common acute lymphoblastic leukemia antigen (CALLA). CD10 is a 100 kDa cell surface molecule identical to human membrane-associated neutral endopeptidase (NEP) and also known as neprilysin or enkephalinase. Human CD10 is expressed on a wide variety of normal and neoplastic cell types from different tissues including neural and hematopoietic cells. It is expressed on pre– and pro–B cells and is involved in B cell development and differentiation. The antigen is also present on mature neutrophils, T cell precursors, and some T cell leukemias/lymphomas. Furthermore, CD10 is expressed on neoplastic cells of several B lymphoid leukemias/lymphomas.

Applications

Reagent can be used for immunophenotyping by flow cytometry. Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. It is important to understand the normal expression pattern for this antigen and its relationship to expression of other relevant antigens in order to perform appropriate analysis.
Expression of CD10 may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Alternative names

CALLA, gp100, NEP

Technical specifications

  • Antigen: CD10
  • Clone: 97C5
  • Isotype: mouse IgG1
  • Alternative names of antigen: CALLA, gp100, NEP
  • Molecular mass of antigen [kDa]: 85
  • Distribution of antigen: B cells, bone marrow, brain, epithelial cells, fibroblasts, granulocytes, gut, kidney, leukemia cells, ES and iPS cells, smooth muscle, stromal cells, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC
  • Selected references

    1. Shipp, M. A. and Look, A. T. (1993) Hematopoietic differentiation antigens that are membrane-associated enzymes: cutting is the key! Blood 82: 1052-1070
    2. Kalled, S. L. et al. (1995) The distribution of CD10 (NEP 24.11, CALLA) in human and mice is similar in non-lymphoid organs but differ within the hematopoietic system: absence on murine T and B lymphoid progenitors. Eur. J. Immunol. 25: 677-687
    3. Wood BL et al. (2007) 2006 Bethesda International Consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry: optimal reagents and reporting for the flow cytometric diagnosis of hematopoietic neoplasia. Cytometry B Clin. Cytom. 72: 14-22
    4. Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
    5. Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
    6. van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
    7. Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
    8. Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of immunologically defined cell populations by flow cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition
    9. Kishimoto, T et al. (eds) Leucocyte typing VI: White cell differentiation antigens. Garland Publishing Inc., New York
  • Brochures and posters

  • Certificates

    Please follow this
    link
    to download the Certificate of Conformity (CoC) by lot number.
Product options: 2
170-078-008

CD10-PE

for 100
tests
DKK 3.555,00 
170-078-009

CD10-APC

for 100
tests
DKK 3.555,00