Clone:
DG3
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC, MICS, IF, IHC
Alternative names:
Thy-1, CDw90

Extended validation for CD90 Antibody, anti-human

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD90. HEL 92.1.7 cells were stained with CD90 antibodies and with a suitable counterstaining. As a control, CD90 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD90 (DG3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD90 (DG3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD90 (DG3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD90 Antibody, anti-human

Overview

CD90 is a 25–35 kD GPI-anchored protein of the Ig superfamily. It is expressed on neurons, small subsets of human fetal liver cells and thymocytes, cord blood, and bone marrow cells. CD90 is also expressed on a subset of CD34
+
primitive hematopoietic stem cells. CD90
+
CD34
+
cells characterize a highly enriched population of high proliferative potential colony-forming cells (HPP-CFC). Furthermore, CD90 expression is found on mesenchymal stromal cells (MSCs). CD90 is involved in regulation of adhesion and signal transduction of T cells.

Alternative names

Thy-1, CDw90

Detailed product information

Technical specifications

CloneDG3
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD90
Alternative names of antigenThy-1, CDw90
Molecular mass of antigen [kDa]13
Distribution of antigenendothelial cells, fibroblasts, T cells, stem cells, leukocytes, lymphocytes, hematopoietic stem and progenitor cells, leukemia cells, mesenchymal stem cells, ES and iPS cells, transitional B cells, brain
Entrez Gene ID7070
RRIDAB_2784296, AB_2733846, AB_2733847, AB_2819380, AB_2819379, AB_2784299, AB_2784298, AB_2751955, AB_2751908, AB_2751954, AB_2751907, AB_2784295, AB_2784294, AB_2784297

Resources for CD90 Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

Reviews for CD90 Antibody, anti-human

Excellent CD90 Antibody from Miltenyi Biotec

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  • 5

CD90-FITC, human (130-117-684)

The antibody produced very accurate staining. Reliable results, easy to use.

References for CD90 Antibody, anti-human

Publications

  1. Mayani, H. and Lansdorp, P. M. (1994) Thy-1 expression is linked to functional properties of primitive hematopoietic progenitor cells from human umbilical cord blood. Blood 83: 2410-2417
  2. Dominici, M. et al. (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8: 315-317

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