The CD8
+
T Cell Isolation Kit has been developed for fast isolation of untouched cytotoxic CD8
+
T cells from human peripheral blood mononuclear cells (PBMCs). This updated kit offers even better performance and a significantly shorter protocol and replaces the previous kit (#130-094-156).

Data and images for
CD8
+
T Cell Isolation Kit
, human

Figures

Figure 1

Untouched CD8
+
T cells were isolated from human PBMCs using the CD8
+
T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD8-FITC, CD56-PE, and CD3-APC to visualize the target cell fraction. Isolated CD8
+
T cells were CD3
+
and CD56
. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer.
Unseparated fraction
View details

Figure 1

Untouched CD8
+
T cells were isolated from human PBMCs using the CD8
+
T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD8-FITC, CD56-PE, and CD3-APC to visualize the target cell fraction. Isolated CD8
+
T cells were CD3
+
and CD56
. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer.
View details

Figure 1

Untouched CD8
+
T cells were isolated from human PBMCs using the CD8
+
T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD8-FITC, CD56-PE, and CD3-APC to visualize the target cell fraction. Isolated CD8
+
T cells were CD3
+
and CD56
. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer.
Isolated CD8+ T cells
View details

Figure 1

Untouched CD8
+
T cells were isolated from human PBMCs using the CD8
+
T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD8-FITC, CD56-PE, and CD3-APC to visualize the target cell fraction. Isolated CD8
+
T cells were CD3
+
and CD56
. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer.
View details

Figure 1

Untouched CD8
+
T cells were isolated from human PBMCs using the CD8
+
T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD8-FITC, CD56-PE, and CD3-APC to visualize the target cell fraction. Isolated CD8
+
T cells were CD3
+
and CD56
. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer.

Figure 2

View details

Figure 2

Specifications for
CD8
+
T Cell Isolation Kit
, human

Overview

The CD8
+
T Cell Isolation Kit has been developed for fast isolation of untouched cytotoxic CD8
+
T cells from human peripheral blood mononuclear cells (PBMCs). This updated kit offers even better performance and a significantly shorter protocol and replaces the previous kit (#130-094-156).

Detailed product information

Detailed separation procedure

Non-CD8
+
cells, i.e., CD4
+
T cells, monocytes, neutrophils, eosinophils, B cells, stem cells, dendritic cells, NK cells, granulocytes, γ/δ T cells, or erythroid cells are labeled by using a cocktail of biotin-conjugated antibodies. The cocktail contains antibodies against CD4, CD15, CD16, CD19, CD34, CD36, CD56, CD123, TCRγ/δ, and CD235a (Glycophorin A). Subsequently, non-target cells are magnetically labelled with the CD8
+
T Cell MicroBead Cocktail. Isolation of highly pure T cells is achieved by depletion of magnetically labelled cells.

Downstream applications

The CD8
+
T Cell Isolation Kit is the tool of choice when direct labeling of CD8
+
T cell surface molecules could interfere with downstream applications, e.g., in studies on cytotoxic T cell activation
1
. Untouched CD8
+
T cells may also be used for the isolation of specific cytotoxic T cell subsets, such as naive or memory CD8
+
T cells and activated CD8
+
T cells.

Columns

LS or autoMACS
®
Columns.

References for
CD8
+
T Cell Isolation Kit
, human

Publications

  1. Finney, H. M. et al. (2004) Activation of resting human primary T cells with chimeric receptors: costimulation from CD28, inducible costimulator, CD134, and CD137 in series with signals from the TCR zeta chain. J. Immunol. 172: 104-113

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