CD24 MicroBead Kit, human

Name: CD24 MicroBead Kit, human
Availability: InStock

CD24 MicroBead Kit, human

The CD24 MicroBead Kit has been developed and optimized for the depletion of CD24-expressing cells from cancer cell lines or single-cell suspensions created from solid tissues. It can also be used for positive selection of cancer stem cells from cancer cell lines or single-cell suspensions created from solid tissues.

Product data and images for CD24 MicroBead Kit, human

Figures

Figure 1

CD24

⁺

cells were depleted from the CML cell line K562 using the CD24 MicroBead Kit, an LD Column, and a QuadroMACS™ Separator. Cells were fluorescently labeled with CD24-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

A:	B:
Unseparated fraction	CD24 ^- cell fraction
View details CD24 MicroBead Kit, human Figure 1 CD24 ⁺ cells were depleted from the CML cell line K562 using the CD24 MicroBead Kit, an LD Column, and a QuadroMACS™ Separator. Cells were fluorescently labeled with CD24-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.	View details CD24 MicroBead Kit, human Figure 1 CD24 ⁺ cells were depleted from the CML cell line K562 using the CD24 MicroBead Kit, an LD Column, and a QuadroMACS™ Separator. Cells were fluorescently labeled with CD24-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD24 MicroBead Kit, human

Overview

The CD24 MicroBead Kit has been developed and optimized for the depletion of CD24-expressing cells from cancer cell lines or single-cell suspensions created from solid tissues. It can also be used for positive selection of cancer stem cells from cancer cell lines or single-cell suspensions created from solid tissues.

Detailed product information

Columns

For depletion: LD Columns for optimal depletion efficiency; MS or LS Columns for cells which strongly express the CD24 antigen; autoMACS

^®

Columns.

For positive selection: MS, LS, or autoMACS Columns.

Resources for CD24 MicroBead Kit, human

Documents and Protocols

Brochures/posters

Cancer Research - Understanding cancer begins with the cell

Scientific posters

Magnetic isolation and rare cell gene expression analysis of breast cancer stem cells

Scientific posters

Isolation of tumor cell subpopulations using semi-automated tissue dissociation and magnetic cell separation

References for CD24 MicroBead Kit, human

Publications

Al-Hajj, M. et al. (2003) Prospective identification of tumorigenic breast cancer cells. Proc. Natl. Acad. Sci. U.S.A. 100: 3983-3988
Gao, M. Q. et al. (2010)
CD24
⁺
cells from hierarchically organized ovarian cancer are enriched in cancer stem cells.
Oncogene 29: 2672-2680
Li, C. P. et al. (2007) Identification of pancreatic cancer stem cells. Cancer Res. 67: 1030-1037

Data and images

Data and images for CD24 MicroBead Kit, human

Figures

Figure 1

CD24

⁺

cells were depleted from the CML cell line K562 using the CD24 MicroBead Kit, an LD Column, and a QuadroMACS™ Separator. Cells were fluorescently labeled with CD24-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

A:	B:
Unseparated fraction	CD24 ^- cell fraction
View details CD24 MicroBead Kit, human Figure 1 CD24 ⁺ cells were depleted from the CML cell line K562 using the CD24 MicroBead Kit, an LD Column, and a QuadroMACS™ Separator. Cells were fluorescently labeled with CD24-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.	View details CD24 MicroBead Kit, human Figure 1 CD24 ⁺ cells were depleted from the CML cell line K562 using the CD24 MicroBead Kit, an LD Column, and a QuadroMACS™ Separator. Cells were fluorescently labeled with CD24-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications

Specifications for CD24 MicroBead Kit, human

Overview

The CD24 MicroBead Kit has been developed and optimized for the depletion of CD24-expressing cells from cancer cell lines or single-cell suspensions created from solid tissues. It can also be used for positive selection of cancer stem cells from cancer cell lines or single-cell suspensions created from solid tissues.

Detailed product information

Columns

For depletion: LD Columns for optimal depletion efficiency; MS or LS Columns for cells which strongly express the CD24 antigen; autoMACS

^®

Columns.

For positive selection: MS, LS, or autoMACS Columns.

Resources

Resources for CD24 MicroBead Kit, human

Documents and Protocols

Brochures/posters

References for CD24 MicroBead Kit, human

Publications

Al-Hajj, M. et al. (2003) Prospective identification of tumorigenic breast cancer cells. Proc. Natl. Acad. Sci. U.S.A. 100: 3983-3988
Gao, M. Q. et al. (2010)
CD24
⁺
cells from hierarchically organized ovarian cancer are enriched in cancer stem cells.
Oncogene 29: 2672-2680
Li, C. P. et al. (2007) Identification of pancreatic cancer stem cells. Cancer Res. 67: 1030-1037

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CD24 MicroBead Kit, human

CD24 MicroBead Kit, human

Product data and images for CD24 MicroBead Kit, human

Figures

Figure 1