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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4+
An appropriate threshold was defined based on CD45-VioBlue versus side scatter signals, for the exclusion of debris and erythrocytes from the data.
Lymphocytes were gated for their expected morphology using a forward scatter versus side scatter "lymphocyte gate" (P1)
Dead cells were excluded by a CD127-PE versus propidium iodide dot plot (P2).
lymphocytes were gated in P3.
Treg cells were gated by a CD127-PE versus CD25-APC dot plot.