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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Whole blood from a healthy donor was stained with the 8-Color Immunophenotyping Kit, human. Staining was carried out at room temperature for 10 minutes. Subsequently, red blood cells were lysed by incubation using 1× Red Blood Cell Lysis Solution at room temperature for 15 minutes. Cells were analyzed by flow cytometry using the MACSQuant®
As a preliminary step for elimination of doublets a gate around single cells in forward scatter area (FSC-A) versus forward scatter height (FSC-H) (A) as well as a gate around viable cells was set (B). To identify the major circulating blood cell types CD45 was used to target all leukocytes (C). Theses cells were further separated from debris via forward scatter (FSC) and side scatter (SSC) (D). Monocytes were discriminated based on their CD14 expression (E) and then further divided into classical, intermediate, and non-classical monocytes via CD16 (F). Among the non-monocyte population, B cells were defined as CD19+
(G). The remaining cells were separated into CD16+
eosinophils as well as a CD16–/dim
population (H). CD3 and CD56 were used to distinguish CD56+
NK cells, CD3+
T cells and a CD3+
T cell population (I). The T cells were divided into CD4+
T cells (J).