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Miltenyi Biotec Inc.
2303 Lindbergh Street
Auburn, CA 95602
USA
Phone: +1 800 FOR MACS
+1 530 888 8871
Fax: +1 530 888 8925
E-Mail: macs@miltenyibiotec.com
Web: www.miltenyibiotec.com
For immediate technical support, use our live chat.
Connect with usOrders:
Phone: +1 866 811 4466
Fax: +1 877 591 1060
E-Mail: orderdesk@miltenyibiotec.com
Technical support - Research products:
Phone: +1 800 FOR MACS
Fax: +1 530 745 2806
E-Mail: science@miltenyibiotec.com
Technical support - Clinical products:
Phone: +1 800 FOR MACS
Fax: +1 530 745 2806
E-Mail: science@miltenyibiotec.com
Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Our local employees are always happy to answer your questions. Highly trained and experienced teams in your country can provide quick, helpful, and comprehensive support.
Miltenyi Biotec Inc.
2303 Lindbergh Street
Auburn, CA 95602
USA
Phone: +1 800 FOR MACS
+1 530 888 8871
Fax: +1 530 888 8925
E-Mail: macs@miltenyibiotec.com
Web: www.miltenyibiotec.com
For immediate technical support, use our live chat. Connect with us
Orders
Phone: +1 866 811 4466
Fax: +1 877 591 1060
E-Mail: orderdesk@miltenyibiotec.com
Technical support - Research products
Phone: +1 800 FOR MACS
Fax: +1 530 745 2806
E-Mail: science@miltenyibiotec.com
Technical support - Clinical products
Phone: +1 800 FOR MACS
Fax: +1 530 745 2806
E-Mail: science@miltenyibiotec.com
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Fluorochrome | Excitation | Exmax (nm) | Emmax (nm) | MACSQuant Analyzer | MACSQuant VYB | ||
laser (nm) | Channel | Filter (nm) | Channel | Filter (nm) | |||
VioBlue | 405 | 400 | 452 | V1 | 450/50 | V1 | 450/50 |
VioGreen | 405 | 388 | 520 | V2 | 525/50 | V2 | 525/50 |
VioBright 515 | 488 | 488 | 514 | B1 | 525/50 | B1 | 525/50 |
Vio 515 | 488 | 488 | 514 | B1 | 525/50 | B1 | 525/50 |
VioBright FITC | 488 | 496 | 522 | B1 | 525/50 | B1 | 525/50 |
FITC | 488 | 495 | 520 | B1 | 525/50 | B1 | 525/50 |
PE | 488 or 561 | 565 | 578 | B2 | 585/40 | Y1 | 586/15 |
PE-Vio 615 | 488 or 561 | 565 | 619 | B3 | 655-730 | Y2 | 615/20 |
PerCP | 488 | 482 | 675 | B3 | 655-730 | N/A | N/A |
PerCP-Vio 700 | 488 | 482 | 704 | B3 | 655-730 | N/A | N/A |
PE-Vio 770 | 488 or 561 | 565 | 775 | B4 | 750 LP | Y4 | 750 LP |
APC | 561 or 635 | 652 | 660 | R1 | 655-730 | Y3 | 661/20 |
APC-Vio 770 | 561 or 635 | 652 | 775 | R2 | 750 LP | Y4 | 750 LP |
Vio 667 | 561 or 635 | 645 | 668 | R1 | 655–730 | Y3 | 661/20 |
Vio Bright 667 | 561 or 635 | 645 | 668 | R1 | 655–730 | Y3 | 661/20 |
Designed to maximize the potential of a flow cytometer’s violet laser, the VioBlue® Dye shows superior performance compared to many other fluorophores excited at 405 nm, including significant advances in brightness, signal-to-noise ratios, and intralaser compensation requirements. In addition, VioBlue exhibits minimal photo-induced degradation, and can consequently be used for many different applications, such as fluorescence microscopy.
VioBlue Dye at a glance:
For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
Fluorochrome | Absorption max. (nm) | Emission max. (nm) |
---|---|---|
VioBlue | 400 | 455 |
Pacific Blue | 405 | 455 |
Cascade Blue | (375); 401 | 423 |
Alexa Fluor 405 | 405 | 421 |
eFluor 405 | 405 | 450 |
BD Horizon V450 | 404 | 448 |
Designed to maximize the potential of a flow cytometer’s violet laser, the VioGreen™ Dye shows superior performance compared with many other fluorophores excited at 405 nm, including significant advances in brightness, signal-to-noise ratios, and intralaser compensation requirements. In addition, VioGreen exhibits minimal levels of photo-induced degradation, and can consequently be used for many different applications, such as fluorescence microscopy.
VioGreen Dye at a glance:
For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
Fluorochrome | Absorption max. (nm) | Emission max. (nm) |
---|---|---|
VioGreen | 388 | 520 |
Pacific Orange | 400 | 551 |
Krome Orange | 398 | 528 |
AmCyan | 458 | 489 |
HorizonV500 | 415 | 500 |
VioBright 515 and Vio 515 are extremely bright dyes for the B1/FITC-channel. The proprietary VioBright multimerization technology results in a 4-fold increase in brightness over the traditional FITC dye. With a 25% reduced spill-over into the B2/PE-channel and high photo stability, these dyes are excellent choices for immunofluorescence microscopy applications.
VioBright 515 at a glance:
Vio 515 at a glance:
For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
VioBright FITC is a blue laser (488 nm) excited revolutionary dye, which allows an increased number of FITC molecules per antibody, as compared to conventional FITC conjugation. With brightness similar to PE, VioBright FITC expands the dimensions of multicolor flow analysis. In addition, it provides a bright alternative for confident detection of rare cells, as well as dim and uncharacterized markers.
VioBright FITC at a glance:
For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
Absorption and emission peaks of fluorochromes compared to VioBright 515, Vio 515, and VioBright FITC.
Fluorochrome | Absorption max. (nm) | Emission max. (nm) |
---|---|---|
VioBright 515 | 488 | 514 |
Vio 515 | 488 | 514 |
VioBright FITC | 496 | 522 |
FITC | 495 | 520 |
Alexa Fluor 488 | 495 | 519 |
BD Horizon Brilliant Blue 515 | 490 | 515 |
The PerCP-Vio 700 Dye is a tandem conjugate that combines the peridinin chlorophyll protein (PerCP) and the new Vio 700 Dye to emit a strong fluorescence at 655–730 nm upon blue laser excitation at 488 nm. This dye is suited perfectly for the B3 channel of the MACSQuant® Analyzer.
For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
Absorption and emission maximums of fluorochromes comparable to PerCP-Vio 700.
Fluorochrome | Absorption max. (nm) | Emission max. (nm) |
---|---|---|
PerCP | 482 | 675 |
PerCP-Vio 700 | 482 | 704 |
PerCP-Cy5.5 | 490 | 695 |
For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
Absorption and emission maximums of fluorochromes comparable to PE-Vio 770.
Fluorochrome | Absorption max. (nm) | Emission max. (nm) |
---|---|---|
PE-Vio 770 | 565 | 775 |
PE-Cy7 | 496 | 774 |
PE-Alexa Fluor 750 | 496 | 775 |
For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
Absorption and emission maximums of fluorochromes comparable to PE-Vio 615.
Fluorochrome | Absorption max. (nm) | Emission max. (nm) |
PE-Vio 615 | 565 | 619 |
ECD, PE-Texas Red | 565 | 613 |
PE-efluor 610 | 565 | 606 |
PE-CF594 | 565 | 614 |
PE/Dazzle 594 | 566 | 612 |
For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
Absorption and emission maxima of fluorochromes related to APC-Vio 770.
Fluorochrome | Absorption max. (nm) | Emission max. (nm) |
---|---|---|
APC-Vio 770 | 625 | 775 |
APC-Cy7 | 650 | 774 |
APC-Alexa Fluor | 750 | 775 |
VioBright 667 and Vio 667 are extremely bright dyes for the R1/APC-channel. The proprietary VioBright multimerization technology results in a 2-fold increase in brightness over the traditional APC dye.
VioBright 667 at a glance:
Vio 667 at a glance:
For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
Absorption and emission maxima of fluorochromes related to APC.
Fluorochrome | Absorption max. (nm) | Emission max. (nm) |
---|---|---|
VioBright 667 | 645 | 668 |
Vio 667 | 645 | 668 |
APC | 650 | 660 |