Bright and stable fluorochromes for multicolor flow cytometry

VioBlue® Dye (Em_max 452 nm)

Designed to maximize the potential of a flow cytometer’s violet laser, the VioBlue® Dye shows superior performance compared to many other fluorophores excited at 405 nm, including significant advances in brightness, signal-to-noise ratios, and intralaser compensation requirements. In addition, VioBlue exhibits minimal photo-induced degradation, and can consequently be used for many different applications, such as fluorescence microscopy.

VioBlue Dye at a glance:

• Coumarin-based dye with excitation and emission wavelengths of 400 nm and 455 nm, respectively
  
• Brighter alternative to Pacific Blue™, Alexa Fluor® 405, or BD Horizon™ V450
  
• Multiplexing of VioBlue with other fluorochromes is easily possible, adding to the variety of marker combination for multiparameter flow cytometry
  
• Combined use with the VioGreen Dye

For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.

VioGreen™ Dye (Em_max 520 nm)

Designed to maximize the potential of a flow cytometer’s violet laser, the VioGreen™ Dye shows superior performance compared with many other fluorophores excited at 405 nm, including significant advances in brightness, signal-to-noise ratios, and intralaser compensation requirements. In addition, VioGreen exhibits minimal levels of photo-induced degradation, and can consequently be used for many different applications, such as fluorescence microscopy.

VioGreen Dye at a glance:

• Large Stokes shift fluorophore, emitting strong fluorescence at 520 nm upon excitation at 405 nm
  
• Significantly increased mean fluorescence intensities and higher stain indices than Pacific Orange™, Krome Orange™, and BD Horizon™ V500
  
• Non-protein fluorophore
  
• Combined use with the VioBlue Dye
  
• Perfectly suited for multiparameter flow cytometry using all current flow cytometer
  

For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.

VioBright™ 515 and Vio 515 (Em_max 514 nm)

VioBright 515 and Vio 515 are extremely bright dyes for the B1/FITC-channel. The proprietary VioBright multimerization technology results in a 4-fold increase in brightness over the traditional FITC dye. With a 25% reduced spill-over into the B2/PE-channel and high photo stability, these dyes are excellent choices for immunofluorescence microscopy applications.

VioBright 515 at a glance:

• Brightest Vio Dye for the blue laser
  
• Excellent choice for surface markers, in particular for low expressed antigens
  

Vio 515 at a glance:

• Brighter alternative to FITC
• Perfectly suited for intracellular markers, such as cytokines and transcription factors
  

For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.

VioBright™ FITC (Em_max 522 nm)

VioBright FITC is a blue laser (488 nm) excited revolutionary dye, which allows an increased number of FITC molecules per antibody, as compared to conventional FITC conjugation. With brightness similar to PE, VioBright FITC expands the dimensions of multicolor flow analysis. In addition, it provides a bright alternative for confident detection of rare cells, as well as dim and uncharacterized markers.

VioBright FITC at a glance:

• Brightness similar to PE for confident detection of low-expressed and rare markers
  
• Excellent bright alternative to PE for flexible multicolor panel design
  
• Signal detectable in standard FITC channel with peak excitation and emission wavelength of 496 nm and 522 nm, respectively
  
• Works with your standard staining protocol

For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.

PerCP-Vio 700 (Em_max 704 nm)

The PerCP-Vio 700 Dye is a tandem conjugate that combines the peridinin chlorophyll protein (PerCP) and the new Vio 700 Dye to emit a strong fluorescence at 655–730 nm upon blue laser excitation at 488 nm.  This dye is suited perfectly for the B3 channel of the MACSQuant® Analyzer.

For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.

PE-Vio 770 (Em_max 770 nm)

• Excitation with the blue (488 nm) or yellow (561 nm) laser, emission in the near-infrared region at 775 nm.
  
• The combination of Vio 770 as the acceptor dye and an optimized chemistry have furnished a tandem dye that is characterized by a high fluorescence intensity, minimal spillover to adjacent detection channels, and low non-specific binding to non-target cells to meet the complexity of multiparameter flow cytometry.
  
• Higher MFI and stain index values, in addition to lower compensation settings when compared to PE-Cy7

For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.

PE-Vio 615 (Em_max 619 nm)

• Optimal excitation with blue (488 nm), green (532 nm), and yellow green (561 nm) laser lines for maximum flexibility. 
  
• Excellent brightness for confident detection of dim, rare, and uncharacterized markers.
  
• Extensive portfolio of PE-Vio 615 dye conjugated to recombinantly engineered REAfinity™ Antibodies for higher reproducibility.
  

For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.

APC-Vio 770 (Em_max 770 nm)

• Excitation with the yellow (561 nm) or red (635 nm) laser, emission in the near-infrared region at 775 nm.
  
• The combination of Vio 770 as the acceptor dye and an optimized chemistry have furnished a tandem dye that is characterized by a high fluorescence intensity, minimal spillover to adjacent detection channels, and low non-specific binding to non-target cells to meet the complexity of multiparameter flow cytometry.
  
• Higher MFI and stain index values, in addition to lower compensation settings when compared to APC-Cy7.
  

For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.

VioBright 667 and Vio 667 (Em_max 668 nm)

VioBright 667 and Vio 667 are extremely bright dyes for the R1/APC-channel. The proprietary VioBright multimerization technology results in a 2-fold increase in brightness over the traditional APC dye. 

 

VioBright 667 at a glance:

• Brighter alternative to APC
• Brightest Vio Dye for the red laser
• Excellent choice for surface markers, in particular for low expressed antigens

Vio 667 at a glance:

• As bright as APC
• Perfectly suited for intracellular markers, such as cytokines and transcription factors

For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
 

• Fluorescence spectra overview poster
• Fluorochromes and instruments poster
• Fluorochrome brightness index
• MACS Antibodies – Quality assured from lot to lot
• Tandem Signal Enhancer