CD86 antibodies, mouse

CD86 antibodies, mouse

Clone: PO3.3 | Dilution: 1:50
Antibody clone PO3.3 detects the CD86 antigen, also known as B7-2, an 80 kDa molecule and a member of the immunoglobulin superfamily. Together with CD80 (B7-1) it belongs to the B7 family of costimulatory molecules. CD86 is expressed on activated antigen-presenting cells, including B cells, dendritic cells, and monocytes/macrophages. The interaction of CD86 with its ligands CD28 and CD152 (CTLA- 4) plays a critical role in induction and regulation of immune responses, e.g., cross-talk between T and B cells, T cell costimulation, or immunoglobulin class-switching. Binding of CD86 to CD28 on T cells results in transduction of costimulatory signals for activation or proliferation of T cells, or cytokine production. In contrast, binding of CD86 to CTLA-4 regulates T cell activation and diminishes the immune response.

Alternative names

B7-2, B70, CLS1, Cd28l2, ETC-1, Ly-58, MB7-2, TS/A-2

Technical specifications

  • Antigen: CD86
  • Clone: PO3.3
  • Isotype: rat IgG2bκ
  • Isotype control: Rat IgG2b – isotype control antibodies
  • Alternative names of antigen: B7-2, B70, CLS1, Cd28l2, ETC-1, Ly-58, MB7-2, TS/A-2
  • Entrez Gene ID: 12524
  • Molecular mass of antigen [kDa]: 32
  • Distribution of antigen: B cells, dendritic cells, endothelial cells, Langerhans cells, monocytes, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC-Vio770, VioBlue
  • Selected references

    1. Kin, N. W. et al. (2006) CD86 stimulation on a B cell activates the phosphatidylinositol 3-kinase/Akt and phospholipase C gamma 2/protein kinase C alpha beta signaling pathways. J. Immunol. 176(11): 6727-6735
    2. Nakajima, A. et al. (1997) Requirement of CD28-CD86 co-stimulation in the interaction between antigen-primed T helper type 2 and B cells. Int. Immunol. 9(5): 637-644
    3. Zhu, X. Y. et al. (2005)
      Blockade of CD86 signaling facilitates a Tʜ2 bias at the maternal-fetal interface and expands peripheral CD4
      +
      CD25
      +
      regulatory T cells to rescue abortion-prone fetuses.
      Biol. Reprod. 72(2): 338-345
Product options: 2
30 µg in 200 µL (1)
EUR 80,00 
30 µg in 200 µL (1)
EUR 85,00