CD65 antibodies, human

CD65 antibodies, human

Clone: REA1128 | Dilution: 1:50
Clone REA1128 recognizes the human CD65 antigen, a fucosylated carbohydrate on the cell surface which is also known as ceramide dodecasaccharide or VIM-2. CD65 is formed by fucosylation of the inner lactosamine unit of alpha-2,3-sialylated polylactosamine by alpha-1,3-fucosyltransferase. It is expressed on all myeloid cells during development, highly on granulocytes, and weakly on monocytes in peripheral blood. The sialylated form of CD65 (designated CD65s) appears when the progenitor antigen CD34 disappears, suggesting that this sialylated carbohydrate antigen marks a turning point in normal myeloid differentiation. CD65 is a critical adhesion molecule for extravascular acute myeloid leukemia cell infiltration.
Additional information: Clone REA1128 displays negligible binding to Fc receptors.

Alternative names

VIM-2

Technical specifications

  • Antigen: CD65
  • Clone: REA1128
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: VIM-2
  • Distribution of antigen: granulocytes, macrophages, monocytes, myeloid cells, myeloid stem cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC
  • Selected references

    1. Lund-Johansen, F. et al. (1992) Activation of human phagocytes through carbohydrate antigens (CD15, sialyl-CD15, CDw17, and CDw65). J. Immunol. 148(10): 3221-3229
    2. Bordessoule, D. et al. (1993) Immunohistological patterns of myeloid antigens: tissue distribution of CD13, CD14, CD16, CD31, CD36, CD65, CD66 and CD67. Br. J. Haematol. 83(3): 370-383
    3. Kniep, B. et al. (1996)
      The CDw65 monoclonal antibodies VIM-8 and VIM-11 bind to the neutral glycolipid V
      3
      FucnLc
      8
      Cer.
      J. Biochem. 119(3): 456-462
Product options: 4
130-119-633

CD65-PE, human

for 30
tests
(1)
EUR 90,00 
130-119-518

CD65-PE, human

for 100
tests
(1)
EUR 220,00 
130-119-634

CD65-APC, human

for 30
tests
(1)
EUR 90,00 
130-119-519

CD65-APC, human

for 100
tests
(1)
EUR 220,00 

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