CD64 antibodies, mouse

CD64 antibodies, mouse

Clone: REA286 | Dilution: 1:10
Clone REA286 recognizes the CD64 antigen, a 72 kDa integral membrane glycoprotein also known as high affinity immunoglobulin gamma Fc receptor I (FcγRI). Structurally CD64 is composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is constitutively expressed on macrophages, monocytes, dendritic cells, and mast cells. Treatment with cytokines like IFN-γ and G-CSF induces CD64 upregulation. CD64 binds monomeric IgG-type antibodies with high affinity and mediates endocytosis, phagocytosis, antibody-dependent cellular toxicity, cytokine release, and superoxide generation.
Additional information: Clone REA286 displays negligible binding to Fc receptors.

Alternative names

FCGR1, FcgammaRI, IGGHAFC, FcR I, FcγRI

Technical specifications

  • Antigen: CD64
  • Clone: REA286
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: FCGR1, FcgammaRI, IGGHAFC, FcR I, FcγRI
  • Entrez Gene ID: 14129
  • Molecular mass of antigen [kDa]: 42
  • Distribution of antigen: dendritic cells, macrophages, monocytes, mast cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC
  • Selected references

    1. Tan, P. S. et al. (2003) Unique monoclonal antibodies define expression of FcgRI on macrophages and mast cell lines and demonstrate heterogeneity among subcutaneous and other dendritic cells. J. Immunol. 170: 2549-2556
    2. Ioan-Facsinay, A. et al. (2002) FcgammaRI (CD64) contributes substantially to severity of arthritis, hypersensitivity responses, and protection from bacterial infection. Immunity 16(3): 391-402
    3. Nimmerjahn, F. et al. (2006) Fcgamma receptors: old friends and new family members. Immunity 24(1): 19-28
Product options: 2
130-103-879

CD64-APC, mouse

9 µg in 300 µL (1)
EUR 48,00 
130-103-809

CD64-APC, mouse

30 µg in 1 mL (1)
EUR 65,00