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Miltenyi Biotec distribution:
Human U937 cells, either left unstimulated (left peak) or stimulated with 20 ng/mL human IFN-γ for 18 hours, were stained with CD353 (SLAMF8) antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Latex beads were coated with recombinant human CD353 protein and then stained with CD353 (SLAMF8) antibodies or with the corresponding REA Control (S) antibodies (left peak). Flow cytometry was performed with the MACSQuant®