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C57BL/6 bone marrow–derived dendritic cells were stained with CD301 (CLEC10A) antibodies or with the corresponding REA Control antibodies (left image) as well as with CD11c antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
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