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Splenocytes from C57BL/6 mice were stained with CD25 antibodies or with the corresponding REA Control antibodies (left images) as well as with CD4 antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones.
|Other clones||Overlap in epitope recognition with REA568|