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Peritoneal exudate cells from BALB/c mice were stimulated with IFN-γ for 2 hours, followed by an overnight incubation with LPS. Cells were then stained with CD209b (SIGN-R1) antibodies or with the corresponding REA Control antibodies (left images) as well as with CD11b antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
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