CD164 antibodies, human

CD164 antibodies, human

Clone: 67D2 | Dilution: 1:50
Clone 67D2 recognizes the human CD164 antigen, a single-pass type I membrane protein, which is also known as sialomucin core protein 24, multi-glycosylated core protein 24 (MUC-24), or endolyn. CD164 is expressed by subpopulations of CD34
+
cells. These include the majority of clonogenic myeloid and erythroid progenitors and the hierarchically more primitive precursors. Biochemical and functional characterization of CD164 indicate that this protein exists as a homodimeric molecule of approximately 160 kDa that participates in the adhesion of CD34
+
cells to bone marrow stroma and functions as a signaling molecule, inhibiting the recruitment of primitive hematopoietic cells into cell cycle. CD164 may also play an important role in prostate cancer metastasis and the infiltration of bone marrow by cancer cells.

Alternative names

MGC-24, MUC-24, Endolyn

Technical specifications

  • Antigen: CD164
  • Clone: 67D2
  • Isotype: mouse IgG1κ
  • Isotype control: Mouse IgG1 – isotype control antibodies
  • Alternative names of antigen: MGC-24, MUC-24, Endolyn
  • Entrez Gene ID: 8763
  • Molecular mass of antigen [kDa]: 19
  • Distribution of antigen: myeloid cells, other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC-Vio770

  • Figure 1

    Human peripheral blood mononuclear cells (PBMCs) were stained with CD164 antibodies or with the corresponding isotype control antibodies (left peak) and analyzed by flow cytometry using the MACSQuant
    ®
    Analyzer. CD14
    +
    cells were pre-gated for the analysis. The FcR Blocking Reagent has been used to avoid Fc receptor-mediated antibody labeling. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  • Selected references

    1. Forde, S. et al. (2007)
      Endolyn (CD164) modulates the CXCL12-mediated migration of umbilical cord blood CD133
      +
      cells
      Blood 109: 1825-1833
    2. Zannettino, A. C. et al. (1998) The sialomucin CD164 (MGC-24v) is an adhesive glycoprotein expressed by human hematopoietic progenitors and bone marrow stromal cells that serves as a potent negative regulator of hematopoiesis. Blood 92(8): 2613-2628
    3. Huang, A. F. et al. (2013) CD164 regulates the tumorigenesis of ovarian surface epithelial cells through the SDF-1α/CXCR4 axis. Mol. Cancer 12(1): 115-115
Product options: 2
130-120-139

CD164-APC-Vio770, human

for 30
tests
(1)
EUR 100,00 
130-120-053

CD164-APC-Vio770, human

for 100
tests
(1)
EUR 240,00 

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