CD164 antibodies, human

Technical specifications

• Antigen: CD164
• Clone: 67D2
• Isotype: mouse IgG1κ
• Isotype control: Mouse IgG1 – isotype control antibodies
• Alternative names of antigen: MGC-24, MUC-24, Endolyn
• Entrez Gene ID: 8763
• Molecular mass of antigen [kDa]: 19
• Distribution of antigen: myeloid cells, other
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: APC-Vio770

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with CD164 antibodies or with the corresponding isotype control antibodies (left peak) and analyzed by flow cytometry using the MACSQuant ® Analyzer. CD14 + cells were pre-gated for the analysis. The FcR Blocking Reagent has been used to avoid Fc receptor-mediated antibody labeling. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

• Selected references

  1. Forde, S. et al. (2007)
     Endolyn (CD164) modulates the CXCL12-mediated migration of umbilical cord blood CD133

     ⁺

     cells
     Blood 109: 1825-1833
  2. Zannettino, A. C. et al. (1998) The sialomucin CD164 (MGC-24v) is an adhesive glycoprotein expressed by human hematopoietic progenitors and bone marrow stromal cells that serves as a potent negative regulator of hematopoiesis. Blood 92(8): 2613-2628
  3. Huang, A. F. et al. (2013) CD164 regulates the tumorigenesis of ovarian surface epithelial cells through the SDF-1α/CXCR4 axis. Mol. Cancer 12(1): 115-115