Clone REA599 recognizes the human CD14 antigen. CD14 is part of the functional heteromeric LPS receptor complex comprised of at least CD14, TLR4, and MD-2. It up-regulates cell surface molecules, including adhesion molecules. CD14 is strongly expressed on most human monocytes and macrophages in peripheral blood, other bodily fluids, and in various tissues such as lymph nodes and spleen. CD14 is weakly expressed on subpopulations of human neutrophils and myeloid dendritic cells.
Additional information: Clone REA599 displays negligible binding to Fc receptors.

Alternative names

LPS R

Technical specifications

  • Antigen: CD14
  • Clone: REA599
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: LPS R
  • Entrez Gene ID: 929
  • Molecular mass of antigen [kDa]: 37
  • Cross-reactivity:
    cynomolgus monkey (
    Macaca fascicularis
    )
    , dog, goat, cat, rabbit, mink, bovine, pig, sheep
  • Distribution of antigen: monocytes, macrophages, spleen
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, VioGreen, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin
  • Selected references

    1. Goyer, S. M. and Ferrero, E. (1987) Biochemical analysis of myeloid antigen and cDNA expression of gp55 (CD14). Oxford, Oxford University Press
    2. Wilson, A. D. et al. (1995)
      Selection of monoclonal antibodies for the identification of lymphocyte surface antigens in the New World primate
      Saguinus oedipus oedipus
      (cotton top tamarin).
      J. Immunol. Methods 178: 195-200
    3. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
    4. Neu, C. et al. (2013) CD14-dependent monocyte isolation enhances phagocytosis of listeria monocytogenes by proinflammatory, GM-CSF-derived macrophages. PLoS One 8(6): e66898
    5. Paccou, J. et al. (2013)
      Determination and modulation of total and surface calcium-sensing receptor expression in monocytes
      in vivo
      and
      in vitro
      .
      PLoS One 8(10): e74800
  • Customer reports and application notes

Product options: 20
130-110-576

CD14-FITC, human

for 30
tests
(1)
EUR 60,00 
130-110-518

CD14-FITC, human

for 100
tests
(1)
EUR 145,00 
130-110-577

CD14-PE, human

for 30
tests
(1)
EUR 80,00 
130-110-519

CD14-PE, human

for 100
tests
(1)
EUR 195,00 
130-110-578

CD14-APC, human

for 30
tests
(1)
EUR 80,00 
130-110-520

CD14-APC, human

for 100
tests
(1)
EUR 195,00 
for 30
tests
(1)
EUR 95,00 
for 100
tests
(1)
EUR 230,00 
for 30
tests
(1)
EUR 95,00 
for 100
tests
(1)
EUR 230,00 
for 30
tests
(1)
EUR 100,00 
for 100
tests
(1)
EUR 240,00 
for 30
tests
(1)
EUR 100,00 
for 100
tests
(1)
EUR 240,00 
for 30
tests
(1)
EUR 100,00 
for 100
tests
(1)
EUR 240,00 
for 30
tests
(1)
EUR 100,00 
for 100
tests
(1)
EUR 240,00 
for 30
tests
(1)
EUR 60,00 
for 100
tests
(1)
EUR 145,00 

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