CD100 antibodies, human

CD100 antibodies, human

Clone: REA316 | Dilution: 1:11
Clone REA316 recognizes the human CD100 antigen, a single-pass type I membrane protein which is also known as semaphorin-4D (SEMA4D). CD100 is strongly expressed in skeletal muscle, and at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung, and pancreas. In the immune system, it is present on peripheral blood lymphocytes, spleen, and thymus, especially interfollicular T cells and B cells in the germinal centers of secondary lymphoid follicles. CD100 is an axon guidance molecule which is secreted by oligodendrocytes and induces growth cone collapse in the central nervous system. By binding plexin B1 receptor it functions as an R-Ras GTPase-activating protein and repels axon growth cones in the central nervous system. In the immune system, CD100 plays a critical role by turning off negative signaling by CD72 and significantly enhancing the effects of CD40 on B cell and dendritic cell responses. During skin damage repairs, SEMA4D interacts with plexin B2 on gamma delta T cells to play a role in the healing process.
Additional information: Clone REA316 displays negligible binding to Fc receptors.

Alternative names

SEMA4D, M-sema-G, SEMAJ, coll-4, A8, BB18, GR3

Technical specifications

  • Antigen: CD100
  • Clone: REA316
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: SEMA4D, M-sema-G, SEMAJ, coll-4, A8, BB18, GR3
  • Entrez Gene ID: 10507
  • Molecular mass of antigen [kDa]: 94
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Distribution of antigen: T cells, B cells, brain, kidney, small intestine, spleen, heart, placenta
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio770, APC-Vio770, Biotin
  • Selected references

    1. Herold, C. et al. (1996) CD100 is associated with CD45 at the surface of human T lymphocytes. Role in T cell homotypic adhesion. J. Immunol. 157(12): 5262-5268
    2. Hall, K. T. et al. (1996) Human CD100, a novel leukocyte semaphorin that promotes B-cell aggregation and differentiation. Proc. Natl. Acad. Sci. U.S.A. 93(21): 11780-11785
    3. Basile, J.R. et al. (2005) Semaphorin 4D/plexin-B1 induces endothelial cell migration through the activation of PYK2, Src, and the phosphatidylinositol 3-kinase-Akt pathway. Mol. Cell. Biol. 25(16): 6889-6898
Product options: 12
for 30
tests
(1)
EUR 75,00 
for 100
tests
(1)
EUR 185,00 
130-104-673

CD100-PE, human

for 30
tests
(1)
EUR 90,00 
130-104-601

CD100-PE, human

for 100
tests
(1)
EUR 220,00 
130-104-674

CD100-APC, human

for 30
tests
(1)
EUR 90,00 
130-104-602

CD100-APC, human

for 100
tests
(1)
EUR 220,00 
for 30
tests
(1)
EUR 100,00 
for 100
tests
(1)
EUR 240,00 
for 30
tests
(1)
EUR 100,00 
for 100
tests
(1)
EUR 240,00 
for 30
tests
(1)
EUR 75,00 
for 100
tests
(1)
EUR 185,00 

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