Anti-TNF-α antibodies, human

Anti-TNF-α antibodies, human

Clone: cA2 | Dilution: 1:10
Tumor necrosis factor alpha (TNF-α) is produced by cells that are involved in inflammatory immune responses. TNF-α is secreted by activated CD4
T cells, monocytes, macrophages, NK cells, and neutrophils.
Anti-TNF-α antibodies have been designed for intracellular staining of TNF-α–producing cells. Cells can be stimulated for TNF-α production, for example, by polyclonal stimulation with mitogens. For induction of TNF-α production by antigen-specific T cells, cells are restimulated with the respective antigen. TNF-α can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, TNF-α–producing cells can be stained intracellularly with Anti-TNF-α antibodies. Staining for surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the TNF-α–producing cells.

Alternative names


Technical specifications

  • Antigen: TNF-α
  • Clone: cA2
  • Isotype: human IgG1
  • Alternative names of antigen: TNF, DIF, TNFSF2
  • Entrez Gene ID: 7124
  • Molecular mass of antigen [kDa]: 26
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    cynomolgus monkey (
    Macaca fascicularis
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC
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