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Lymph node cells from C57BL/6 mice were stained with Anti-TCR Vα3.2 (b, c) antibodies or with the corresponding REA control (S) antibodies (left image), as well as with CD3ε antibodies. Flow cytometry was performed with the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.