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Splenocytes from BALB/c mice were stained with Anti-Ly-49F antibodies as well as with Anti-NK1.1 antibodies and analyzed by flow cytometry using the MACSQuant®
cells were excluded from the analysis. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide or 4',6-diamidino-2-phenylindole (DAPI) fluorescence as in the case of tandem conjugates.
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