Anti-LAP (TGF-β1) antibodies, human

Technical specifications

• Antigen: LAP (TGF-β1)
• Clone: CH6-17E5.1
• Isotype: mouse IgG1κ
• Isotype control: Mouse IgG1 – isotype control antibodies
• Alternative names of antigen: TGFB1, CED, DPD1
• Entrez Gene ID: 7040
• Molecular mass of antigen [kDa]: 28
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: VioBright FITC, PE, Biotin

• Figure 1

  Human PBMCs were either left unstimulated (left image) or stimulated with Treg Expansion CD3/CD28 MACSiBead Particles for 48 hours. The cells were stained with Anti-LAP (TGF-β1) antibodies. LAP-TGF-β1–staining was controlled by incubating cells with unconjugated Anti-LAP (TGF-β1) antibodies prior to labeling with fluorochrome-conjugated antibodies. After that the cells were fixed, permeabilized, and intracellularly stained with Anti-FoxP3‑APC or -PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Gating was performed according to the CD4 expression and side scatter properties of the cells. Cell debris was excluded from the analysis in an fluorescence 3 versus fluorescence 4 dot plot.

• Selected references

  1. Tran, D. Q. et al. (2009)
     GARP (LRRC32) is essential for the surface expression of latent TGF-β on platelets and activated FOXP3

     ⁺

     regulatory T cells.
     Proc. Natl. Acad. Sci. U.S.A. 106: 13445-13450
  2. Tran, D.Q. et al. (2009)
     Selective expression of latency-associated peptide (LAP) and IL-1 receptor type I/II (CD121a/CD121b) on activated human FoxP3

     ⁺

     regulatory T cells allows for their purification from expansion cultures.
     Blood 113: 5125-5133