Anti-LAP (TGF-β1) antibodies, human

Anti-LAP (TGF-β1) antibodies, human

Clone: CH6-17E5.1 | Dilution: 1:11
Clone CH6-17E5.1 reacts with the N-terminal latency-associated peptide (LAP) of the transforming growth factor β1 (TGF-β1) dimer. TGF-β1 belongs to a family of homologous, disulfide-linked, homodimeric proteins. These highly pleiotropic cytokines inhibit proliferation of most cells, but can promote the growth of mesenchymal cells and enhance extracellular matrix formation. The pivotal function of TGF-β1 in the immune system is to mediate immunosuppression and maintain tolerance by regulating lymphocyte proliferation, differentiation, and survival. TGF-β1 is produced by many cell types, but is reported to be most abundant in mammalian platelets and bone. It is secreted predominantly as an inactive latent complex. After proteolytical processing of the TGF-β1 precursor, the resulting N-terminal latency-associated peptide (LAP) remains non-covalently associated with the TGF-β1 dimer. Mature and biologically active TGF-β1 can be released from the complex by action of proteases and/or conformational changes. LAP-TGF-β1 was recently described to be expressed on the cell surface of activated human regulatory T cells.

Alternative names

TGFB1, CED, DPD1

Technical specifications

  • Antigen: LAP (TGF-β1)
  • Clone: CH6-17E5.1
  • Isotype: mouse IgG1κ
  • Isotype control: Mouse IgG1 – isotype control antibodies
  • Alternative names of antigen: TGFB1, CED, DPD1
  • Entrez Gene ID: 7040
  • Molecular mass of antigen [kDa]: 28
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, APC, Biotin

  • Figure 1

    Human PBMCs were either left unstimulated (left image) or stimulated with Treg Expansion CD3/CD28 MACSiBead Particles for 48 hours. The cells were stained with Anti-LAP (TGF-β1) antibodies. LAP-TGF-β1–staining was controlled by incubating cells with unconjugated Anti-LAP (TGF-β1) antibodies prior to labeling with fluorochrome-conjugated antibodies. After that the cells were fixed, permeabilized, and intracellularly stained with Anti-FoxP3‑APC or -PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Gating was performed according to the CD4 expression and side scatter properties of the cells. Cell debris was excluded from the analysis in an fluorescence 3 versus fluorescence 4 dot plot.

  • Selected references

    1. Tran, D. Q. et al. (2009)
      GARP (LRRC32) is essential for the surface expression of latent TGF-β on platelets and activated FOXP3
      +
      regulatory T cells.
      Proc. Natl. Acad. Sci. U.S.A. 106: 13445-13450
    2. Tran, D.Q. et al. (2009)
      Selective expression of latency-associated peptide (LAP) and IL-1 receptor type I/II (CD121a/CD121b) on activated human FoxP3
      +
      regulatory T cells allows for their purification from expansion cultures.
      Blood 113: 5125-5133
Product options: 6
130-106-994

Anti-LAP (TGF-β1)-VioBright FITC, human

for 30
tests
(1)
EUR 100,00 
130-106-930

Anti-LAP (TGF-β1)-VioBright FITC, human

for 100
tests
(1)
EUR 240,00 
130-099-947

Anti-LAP (TGF-β1)-PE, human

for 30
tests
(1)
EUR 90,00 
130-096-666

Anti-LAP (TGF-β1)-PE, human

for 100
tests
(1)
EUR 220,00 
130-096-573

Anti-LAP (TGF-β1)-APC, human

for 100
tests
(1)
EUR 220,00 
130-096-735

Anti-LAP (TGF-β1)-Biotin, human

for 100
tests
(1)
EUR 185,00 

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