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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Naive CD4 + T cells were isolated from human peripheral blood mononuclear cells (PBMCs). Cells were either left unstimulated (left images) or stimulated with PMA (50 ng/mL) and Ionomycin (1 µg/mL) for six hours. After two hours, brefeldin A (1 µg/mL) was added to the stimulated and to the unstimulated cells. Cells were fixed, permeabilized, and intracellularly stained with Anti-IL-17A antibodies as well as with CD3 antibodies. Cells were then analyzed by flow cytometry using the MACSQuant ® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris were excluded from the analysis based on scatter signals. |
In order to compare the epitope specificity of REAfinity Clone REA1063 with other known clones, a competition assay was performed.
Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
Other clones | Overlap in epitope recognition with REA1063 |
CZ8-23G1 | ++ |
BL168 | ++ |
N49-653 | ++ |
eBio64DEC17 | - |
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