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Miltenyi Biotec distribution:
Splenocytes from C57BL/6 mice were either left unstimulated (left peak) or stimulated with 100 ng/mL ovalbumin peptide 257-264 for two hours. Cells were then stained with Anti-H-2Kb/SIINFEKL antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.