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Human peripheral blood mononuclear cells (PBMCs), either left unstimulated (left images) or stimulated with CD3/CD28/IL-2 for 2 days, were surface-stained with Anti-GARP (LRRC32) antibodies. Cells were then fixed and permeabilized using the FoxP3 Staining Buffer Set followed by intracellular staining with Anti-FoxP3 antibodies and analyzed by flow cytometry using the MACSQuant®
cells were pre-gated for the analysis. Cell debris were excluded from the analysis based on scatter signals.
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