Anti-CLIP antibodies, human

Anti-CLIP antibodies, human

Clone: REA296 | Dilution: 1:11
Clone REA296 recognizes MHC class II-associated invariant chain (Ii)-derived peptide (CLIP) complexes. MHC class II αβ heterodimers associate early during biosynthesis with a type II membrane protein, the invariant chain (Ii). The invariant chain serves as a chaperone for MHC II molecules and mediates trafficking to the endosomal pathway. In the endosomal pathway Ii is sequentially degraded, leaving a residual CLIP in the peptide-binding groove of MHC II. In presence of antigen peptide fragments, HLA-DM then binds to the MHC II molecule, releasing CLIP and allowing peptides to bind. REA296 detects HLA class II-positive cells which have impaired HLA-DM activity, and tumor cells that have escaped immuno-surveillance by CD4-positive T cells.
Additional information: Clone REA296 displays negligible binding to Fc receptors.

Alternative names

Class II-associated invariant chain peptide, DHLAG, HLADG, II, Ia-GAMMA, CD74

Technical specifications

  • Antigen: CLIP
  • Clone: REA296
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: Class II-associated invariant chain peptide, DHLAG, HLADG, II, Ia-GAMMA, CD74
  • Entrez Gene ID: 972
  • Molecular mass of antigen [kDa]: 34
  • Distribution of antigen: B cells, dendritic cells, macrophages, monocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio770, Biotin
  • Selected references

    1. van Bergen, J. et al. (1997) Efficient loading of HLA-DR with a T helper epitope by genetic exchange of CLIP. Proc. Natl. Acad. Sci. U.S.A. 94(14): 7499-7502
    2. Wälchli, S. et al. (2014) Invariant chain as a vehicle to load antigenic peptides on human MHC class I for cytotoxic T-cell activation. Eur. J. Immunol. 44(3): 774-784
    3. Bakke, O. et al. (1990) MHC class II-associated invariant chain contains a sorting signal for endosomal compartments. Cell 63(4): 707-716
Product options: 10
for 30
tests
(1)
EUR 80,00 
for 100
tests
(1)
EUR 190,00 
for 30
tests
(1)
EUR 95,00 
for 100
tests
(1)
EUR 225,00 
for 30
tests
(1)
EUR 95,00 
for 100
tests
(1)
EUR 225,00 
for 30
tests
(1)
EUR 100,00 
for 100
tests
(1)
EUR 240,00 
for 30
tests
(1)
EUR 80,00 
for 100
tests
(1)
EUR 190,00 

Related products

2 products available