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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Mouse mammary gland was dissociated using the Adipose Tissue Dissociation Kit, mouse and rat (# 130-105-808) in combination with the gentleMACS Octo Dissociator. Subsequently, tissue‑resident stem and progenitor cells were pre-enriched using the Tissue Stem Cell Pre-Enrichment Kit, mouse. Cells were fluorescently stained with lineage markers (CD31, CD45, Ter-119 conjugated to APC) and Labeling Check Reagent‑VioBlue and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Stem cell-enriched fraction
Lineage cell-enriched fraction