CD8 MicroBeads, human

CD8 MicroBeads, human

CD8 MicroBeads were developed for the positive selection or depletion of human cytotoxic CD8
+
T cells from different cell sources. The most commonly used cell sources are peripheral blood and cord blood. In addition, cytotoxic T cells have also been isolated from lymph nodes, thymus, skin biopsies
1
, and spleen.

Background information

The CD8 antigen forms a complex together with the T cell receptor and acts as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on CD8
+
cytotoxic T cells. The CD8 molecule consists of either an α/β heterodimer or an α/α homodimer. It is expressed strongly on cytotoxic T cells and dimly on a subset of NK cells. CD8 is found on most thymocytes and on about one third of all peripheral blood T cells. CD8
+
cytotoxic T cells play an important role in the killing of virus-infected cells and tumor cells.

Downstream applications

Isolation or depletion of CD8
+
cytotoxic T cells is performed in many different research fields such as infectious diseases, autoimmune diseases, allergy, and asthma, as well as tumor immunology.
Cytotoxic T cells isolated by MACS® Technology remain viable and functional. Therefore, they can be used for further functional studies, such as proliferation assays
2,9
and cytotoxicity assays
2,3
, but also for the analysis of
in vitro
cytokine production
4
. CD8 MicroBeads have also been used for the depletion of CD8
+
T cells from human PBMCs for immune reconstitution experiments in SCID mice.
5
In combination with CD4 MultiSort MicroBeads, CD8 MicroBeads have been used for the isolation of CD4
+
CD8
+
double-positive thymocytes.
8
Other examples include various studies on viral infections, e.g.,
in vitro
infections of CD8
+
cells with HIV
6
, or monitoring of immune abnormalities in children of HIV-infected mothers
7
.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
  • Selected references

    1. Akdis, M. et al. (1999)
      Skin homing (cutaneous lymphocyte-associated antigen-positive) CD8
      +
      T cells respond to superantigen and contribute to eosinophilia and IgE production in atopic dermatitis.
      J. Immunol. 163: 466-475
    2. Parra, E. et al. (1997)
      The role of B7-1 and LFA-3 in costimulation of CD8
      +
      T cells.
      J. Immunol. 158: 637-642
    3. Turner, J. and Dockrell, H M. (1996)
      Stimulation of human peripheral blood mononuclear cells with live
      Mycobacterium bovis
      BCG activates cytolytic CD8
      +
      T cells
      in vitro
      .
      Immunology 87: 339-342
    4. Akdis, A. C. et al. (1995) Cytokine immunotrapping: an assay to study the kinetics of production and consumption or degradation of human interferon-gamma. J. Immunol. Methods 182: 251-261
    5. Walker, W. and Gallagher, G. (1994)
      The
      in vivo
      production of specific human antibodies by vaccination of human-PBL-SCID mice.
      Immunology 83: 163-170
    6. Ennen, J. et al. (1994) CD8+ T lymphocytes of African green monkeys secrete an immunodeficiency virus-suppressing lymphokine. Proc. Natl. Acad. Sci. U.S.A. 91: 7207-7211
    7. Nielsen, S. D. et al. (2001) Impaired progenitor cell function in HIV-negative infants of HIV-positive mothers results in decreased thymic output and low CD4 counts. Blood 98: 398-404
    8. Kutlesa, S. et al. (2002) Developmentally regulated interactions of human thymocytes with different laminin isoforms. Immunology 105: 407-418
    9. Al-Shanti, N. et al. (2004)
      Investigation of alpha nascent polypeptide-associated complex functions in a human CD8
      +
      T cell
      ex vivo
      expansion model using antisense oligonucleotides.
      Immunology 112: 397-403
  • Brochures and posters

  • Customer reports and application notes

Product options: 2
for 1×
10
9
total cells
EUR 520,00 
for 1×
10
9
total cells
EUR 520,00 

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