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Separation of non-mobilized PBMCs using CD133/1 (AC133)-Biotin, Anti-Biotin MicroBeads, MS Columns, and a MiniMACS™ Separator. The cells are fluorescently stained with CD34-FITC and CD133/2 (293C3)-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.
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