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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Isolation of PDCs from PBMCs using the Diamond Plasmacytoid Dendritic Cell Isolation Kit II, an LD Column and a MidiMACS™ Separator, as well as a MiniMACS™ Separator and two MS Columns.
Cells were stained with CD303 (BDCA-2)-APC and CD123-PE. Like CD304 (BDCA-4/Neuropilin-1), the CD303 (BDCA-2) antigen is specifically expressed on PDCs in blood and allows their direct identification.
PBMCs before separation
Enriched PDCs after depletion of non-PDCs
Pure PDCs after positive selection with CD304 (BDCA-4/Neuropilin-1) Diamond MicroBeads