Anti-Mouse IgG MicroBeads

Anti-Mouse IgG MicroBeads

Anti-Mouse IgG MicroBeads, formerly known as Goat Anti-Mouse IgG MicroBeads, recognize the heavy and light chains of all mouse IgG antibodies (IgG1, IgG2a, IgG2b, and IgG3). They also react with light chains of other mouse immunoglobulin classes like IgM, IgA, or IgE. Anti-Mouse IgG MicroBeads are conjugated with goat F(ab')
2
fragments which have been affinity-adsorbed to remove cross-reactivity with human immunoglobulins.

Downstream applications

Anti-Mouse IgG MicroBeads have been used for numerous different cell separations, including tumor cells from patients blood
1,2
, fetal trophoblasts from maternal blood
3
, human T cell subsets
4
, and human NK cells
5
from PBMCs as well as the isolation of chicken osteocytes from chicken calvariae and tibiotarsi. Positive selection of trout thrombocytes
6
, a subpopulation of rat macrophages
7
, and porcine hematopoietic stem cells
8
with Anti-Mouse IgG MicroBeads has been reported, as well as the depletion of B cells from
Xenopus laevis
spleen
9
.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
  • Selected references

    1. Wong et al. (1995) Detection of circulating tumour cells with the magnetic activated cell sorter. Br. J. Surg. 82: 1333-1337
    2. Benez, A. et al. (1999) Detection of circulating melanoma cells by immunomagnetic cell sorting. J. Clin. Lab. Anal. 13: 229-233
    3. van Wijk, I.J. et al. (1996) Enrichment of fetal trophoblast cells from the maternal peripheral blood followed by detection of fetal deoxyribonucleic acid with a nested X/Y polymerase chain reaction. Am. J. Obstet. Gynecol. 174: 871-876
    4. Caux, C. et al. (1996)
      CD34
      +
      hematopoietic progenitors from human cord blood differentiate along two independent dendritic cell pathways in response to GM-CSF
      +
      TNF alpha.
      J. Exp. Med. 184: 695-706
    5. Geiselhart, A. et al. (1997)
      Positive selection of CD56
      +
      lymphocytes by magnetic cell sorting.
      Nat. Immun. 15: 227-233
    6. Hill, D. J. et al. (1999) Trout thrombocytes contain 12- but not 5-lipoxygenase activity. Biochim. Biophys. Acta 1437: 63-70
    7. Massimino, M. L. et al. (1997)
      ED2
      +
      macrophages increase selectively myoblast proliferation in muscle cultures.
      Biochem. Biophys. Res. Commun. 235: 754-759
    8. Layton et al. (2007) Development of an anti-porcine CD34 monoclonal antibody that identifies hematopoietic stem cells. Exp. Hematol. 35: 171-178
    9. Horton et al. (2000) Xenopus NK cells identified by novel monoclonal antibodies. Eur. J. Immunol. 30: 604-613
Product options: 2
for 1×
10
9
total cells
EUR 484,00 
for 5×
10
8
total cells
EUR 275,00