Mouse GM-CSF

Mouse GM-CSF

Recombinant mouse GM-CSF can regulate growth and differentiation of cells of the myeloid lineage such as DCs, granulocytes, monocytes, and macrophages. As a crucial part of the immune/inflammatory path, the hematopoietic cytokine serves as both an activation signal for mature myeloid cells and survival. The recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) is developed for use in functional assays, differentiation studies, and cell culture.

Applications

Mouse GM-CSF can be used for a variety of applications, including:
  • Induction of colony formation of granulocyte/macrophage progenitors in semi-solid medium.
  • In vitro generation of DCs from bone marrow or maturation of CD11c+ splenocytes.
  • Generation of antigen-presenting (DC-like) cells in primary brain cell culture.

Background information

GM-CSF is a hematopoietic growth factor, which is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors. It also functions as a growth factor for erythroid and megakaryocytic precursor cells in conjunction with erythropoietin. GM-CSF is secreted by various cell types including T cells, macrophages, endothelial cells, and fibroblasts in response to inflammatory stimuli and cytokines. In addition, GM-CSF strongly chemoattracts neutrophils and eosinophils and enhances the effector functions of neutrophils and macrophages.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of FDC-P1 cells (NIBSC 91/658)
  • premium grade: ≥ 5×
    10
    7
    U/mg
  • research grade: ≥ 1×
    10
    7
    U/mg
  • Selected references

    1. Ait-Oufella et al. (2010) B cell depletion reduces the development of atherosclerosis in mice. J. Exp. Med. 207: 1579-1587
    2. DeLamarter, J.F. et al. (1985)
      Recombinant murine GM-CSF from
      E. coli
      has biological activity and is neutralized by a specific antiserum.
      EMBO J. 4: 2575-2581
    3. Cantini, G. et al. (2012) Immunotherapy against the radial glia marker GLAST effectively triggers specific antitumor effectors without autoimmunity. Oncoimmunology 1(6): 884-893
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 6
E. coli
10 µg
EUR 138,00 
E. coli
25 µg
EUR 215,00 
E. coli
10 µg
EUR 169,00 
E. coli
25 µg
EUR 272,00 
E. coli
100 µg
EUR 538,00 
E. coli
1000 µg
EUR 2.276,00