Clone:
REA463
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
C6orf190, C6orf207, GASP, SPOT, TSEPA, THMS1, GRB2-associated protein

Specifications for THEMIS Antibody, anti-human, REAfinity™

Overview

Clone REA463 recognizes the human thymocyte-expressed molecule involved in selection (THEMIS) antigen, a 73 kDa signaling protein, that is also known as thymocyte selection pathway associated (TSEPA), signaling phosphoprotein specific for T cells (SPOT), or GRB2-associated protein (GASP). THEMIS is rapidly tyrosine-phosphorylated upon TCR ligation and is required for the differentiation of immature CD4/CD8 double positive thymocytes into mature CD4 or CD8 single positive thymocytes. It is expressed only in the T cell lineage and is first detected in CD4/CD8 double negative thymocytes, reaching maximum levels at the CD4/CD8 double positive stage. Following thymocyte selection, THEMIS expression is decreased in mature CD4 and CD8 single positive thymocytes and peripheral T cells. Themis
−/−
mice show impaired positive selection during thymocyte development and severe reduction of mature thymocytes and peripheral T cells.
Additional information: Clone REA463 displays negligible binding to Fc receptors.

Alternative names

C6orf190, C6orf207, GASP, SPOT, TSEPA, THMS1, GRB2-associated protein

Detailed product information

Technical specifications

CloneREA463
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenTHEMIS
Alternative names of antigenC6orf190, C6orf207, GASP, SPOT, TSEPA, THMS1, GRB2-associated protein
Molecular mass of antigen [kDa]74
Distribution of antigenT cells, thymocytes, T helper cells
Entrez Gene ID387357
RRIDAB_2654132, AB_2654133, AB_2654134, AB_2654131

References for THEMIS Antibody, anti-human, REAfinity™

Publications

  1. Paster, W. et al. (2013) GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. J. Immunol. 190(7): 3749-3756
  2. Paster, W. et al. (2015) A THEMIS: SHP1 complex promotes T-cell survival. EMBO J. 34(3): 393-409
  3. Gascoigne, N. R. et al. (2015) THEMIS: a critical TCR signal regulator for ligand discrimination. Curr. Opin. Immunol. 33: 86-92

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