Ultra high content imaging using the principle of iterative staining cycles

The principle of iterative staining

Miltenyi Biotec’s MICS (multiparameter imaging cell screen) technology enables the simultaneous analysis of hundreds of markers on a single sample based on fluorescence microscopy. It uses the principle of iterative staining with different fluorochrome-conjugated antibodies to acquire microscopy data for a multitude of parameters without harming the sample. The iterative process comprises three main steps that are conducted by the MACSima™ Imaging System in a fully automated manner.

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  1. Samples are stained with fluorochrome-conjugated antibodies  
  2. Epifluorescence images are acquired of the desired sample areas 
  3. The fluorescence signal is erased and the process restarts automatically