Mouse IL-10 Secretion Assay – Detection Kits

Mouse IL-10 Secretion Assay – Detection Kits

The IL-10 Secretion Assay was developed for the sensitive detection of mouse IL-10-secreting cells.

Background information

Interleukin-10 (IL-10) is predominantly secreted by CD4
T cells and antigen-presenting cells, such as monocytes/macrophages and dendritic cells. IL-10 has important suppressive functions on immune responses and is involved in the maintenance of tolerance. IL-10 blocks activation of cytokine synthesis by T
1 cells, activated monocytes, and NK cells. It can stimulate the immunoglobulin production by B cells.

Downstream applications

The Mouse IL-10 Secretion Assay was used for the analysis of the sequential production of cytokines by T helper lymphocytes.
The assay was also used for analysis of IL-10 expression by T
2 cells
, by dendritic cells from mouse spleen
, and by T cells from bronchioalveolar lavages
  • Selected references

    1. Donnelly, S. et al. (2014) Secreted proteins from the helminth Fasciola hepatica inhibit the initiation of autoreactive T cell responses and prevent diabetes in the NOD mouse. PLoS One 9(1)
    2. Topp, M. S. et al. (2014) Distinct and complementary roles for Aspergillus fumigatus-specific Tr1 and Foxp3+ regulatory T cells in humans and mice. Immunol. Cell Biol. 92(8): 659-670
    3. Assenmacher et al. (1998) Sequential production of IL-2, IFN-gamma and IL-10 by individual staphylococcal enterotoxin B-activated T helper lymphocytes. Eur. J. Immunol. 28: 1534-1543
    4. Löhning, M. et al. (2003) Establishment of memory for IL-10 expression in developing T helper 2 cells requires repetitive IL-4 costimulation and does not impair proliferation. Proc. Natl. Acad. Sci. U.S.A. 100: 12307-12312
    5. Edwards, A. D. et al. (2002) Microbial recognition via Toll-like receptor-dependent and -independent pathways determines the cytokine response of murine dendritic cell subsets to CD40 triggering. J. Immunol. 169: 3652-3660
    6. Mc Allister, F. et al. (2004)
      T cytotoxic-1 CD8
      T cells are effector cells against pneumocystis in mice.
      J. Immunol. 172: 1132-1138
  • Special protocols

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