Clone:
H5C6
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC, MICS, IF, IHC
Alternative names:
LAMP-3, ME491, MLA1, OMA81H, Tspan30, LIMP1

Extended validation for CD63 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with H5C6
REA1055++
MEM-259++
Cells were incubated with an excess of purified unconjugated CD63 (H5C6) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD63 Antibody, anti-human

Overview

Clone H5C6 recognizes CD63, a type III glycoprotein. CD63 belongs to the tetraspanin family of proteins, which are characterized by four membrane spanning domains. Tetraspanins via various homotypic and heterotypic interactions regulate the spatial location and trafficking of associated transmembrane partners, eventually regulating and co-ordinating various signaling pathways. CD63, like other tetraspanins, is present in the plasma membrane and membrane of various subcellular compartments such as late endosomes, lysososmes, cytotoxic granules of T lymphocytes, the crystalloid granules of eosinophils, the secretory granules of basophils and mast cells. Interaction partners of CD63 include MHC class II molecules in immature dendritic cells and B lymphocytes, FcεRI in basophils and mast cells, etc.

Alternative names

LAMP-3, ME491, MLA1, OMA81H, Tspan30, LIMP1

Detailed product information

Technical specifications

CloneH5C6
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate
Cross-reactivity
chimpanzee (
Pan troglodytes
)
,
olive baboon (
Papio anubis
)
,
cynomolgus monkey (
Macaca fascicularis
)
,
rhesus monkey (
Macaca mulatta
)
AntigenCD63
Alternative names of antigenLAMP-3, ME491, MLA1, OMA81H, Tspan30, LIMP1
Molecular mass of antigen [kDa]26
Distribution of antigenB cells, granulocytes, eosinophils
Entrez Gene ID967
RRIDAB_2811541, AB_2819561, AB_2819531, AB_2658912, AB_2658913, AB_2658917, AB_2658918, AB_2658919, AB_2658920, AB_2658921, AB_2658922, AB_2811574

References for CD63 Antibody, anti-human

Publications

  1. Mahmudi-Azer, S. et al. (2002) Translocation of the tetraspanin CD63 in association with human eosinophil mediator release. Blood 99: 4039-4047
  2. Vischer, U. M. and Wagner, D. D. (1993) CD63 is a component of Weibel-Palade bodies of human endothelial cells. Blood 82: 1184-1191
  3. Pols, M. S. and Klumperman, J. (2009) Trafficking and function of the tetraspanin CD63. Exp. Cell Res. 315(9): 1584-1592

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