Clone:
REA188
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
Itga2, BR, α2 integrin, gpIa, HPA-5, VLA-2, VLAA2, CD49b, VLA-2α

Extended validation for CD49b Antibody, anti-human, REAfinity™

Sensitivity

View details
Flow cytometric comparison of different clones for CD49b. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD49b antibodies and with a suitable counterstaining. As a control, CD49b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD49b. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD49b antibodies and with a suitable counterstaining. As a control, CD49b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD49b. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD49b antibodies and with a suitable counterstaining. As a control, CD49b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD49b. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD49b antibodies and with a suitable counterstaining. As a control, CD49b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD49b. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD49b antibodies and with a suitable counterstaining. As a control, CD49b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD49b (REA188). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD49b (REA188). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD49b (REA188). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD49b Antibody, anti-human, REAfinity™

Overview

Clone REA188 recognizes CD49b, also known as integrin α2 chain. CD49b is single-pass, 150 KDa, type I membrane protein. Together with integrin β1 (CD29), CD49b forms the VLA-2 receptor, which binds ligands such as collagen, fibrinogen, and laminin and thus mediate the attachment of several cell types to extracellular matrix. Expression of CD49b is found on lymphocytes, activated T cells, monocytes, epithelial cells, and platelets.
Additional information: Clone REA188 displays negligible binding to Fc receptors.

Alternative names

Itga2, BR, α2 integrin, gpIa, HPA-5, VLA-2, VLAA2, CD49b, VLA-2α

Detailed product information

Technical specifications

CloneREA188
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD49b
Alternative names of antigenItga2, BR, α2 integrin, gpIa, HPA-5, VLA-2, VLAA2, CD49b, VLA-2α
Molecular mass of antigen [kDa]126
Distribution of antigenmegakaryocytes, monocytes, platelets, T cells
Entrez Gene ID3673
RRIDAB_2857655, AB_2857630, AB_2658459, AB_2658460, AB_2658463, AB_2658464, AB_2658465, AB_2658466, AB_2658467, AB_2658468, AB_2905054, AB_2905053

Resources for CD49b Antibody, anti-human, REAfinity™

Certificates

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References for CD49b Antibody, anti-human, REAfinity™

Publications

  1. Elices, M. J. and Hemler, M. E. (1989) The human integrin VLA-2 is a collagen receptor on some cells and a collagen/laminin receptor on others. Proc. Natl. Acad. Sci. U.S.A. 86: 9906-9910

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