Clone:
REA607
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
gpIIb/IIIa complex, integrin αIIbβ3

Extended validation for CD41/CD61 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA607
A2A9/6++
PAC-1n/a
Cells were incubated with an excess of purified unconjugated CD41/CD61 (REA607) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD41/CD61. Human peripheral blood mononuclear cells (PBMCs) were stained with CD41/CD61 antibodies and with a suitable counterstaining. As a control, CD41/CD61 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD41/CD61. Human peripheral blood mononuclear cells (PBMCs) were stained with CD41/CD61 antibodies and with a suitable counterstaining. As a control, CD41/CD61 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD41/CD61. Human peripheral blood mononuclear cells (PBMCs) were stained with CD41/CD61 antibodies and with a suitable counterstaining. As a control, CD41/CD61 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD41/CD61. Human peripheral blood mononuclear cells (PBMCs) were stained with CD41/CD61 antibodies and with a suitable counterstaining. As a control, CD41/CD61 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD41/CD61 (REA607). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD41/CD61 (REA607). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD41/CD61 (REA607). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD41/CD61 Antibody, anti-human, REAfinity™

Overview

Clone REA607 recognizes the CD41/CD61 complex, also known as gpIIb/IIIa complex or integrin αIIbβ3. This cell adhesion molecule is a α/β transmembrane heterodimer of the ubiquitous integrin superfamily which is expressed by megakaryocytes and platelets. The CD41/CD61 complex plays an significant role in hemostasis and is also involved in fibrinogen binding and platelet aggregation.
Additional information: Clone REA607 displays negligible binding to Fc receptors.

Alternative names

gpIIb/IIIa complex, integrin αIIbβ3

Detailed product information

Technical specifications

CloneREA607
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD41/CD61
Alternative names of antigengpIIb/IIIa complex, integrin αIIbβ3
Distribution of antigenmegakaryocytes, platelets
Entrez Gene ID3674, 3690
RRIDAB_2751734, AB_2802027, AB_2802010, AB_2802037, AB_2802025, AB_2889578, AB_2889577, AB_2819707, AB_2819699, AB_2877032, AB_2877033, AB_2658032, AB_2658033, AB_2658036, AB_2658037, AB_2751794

Resources for CD41/CD61 Antibody, anti-human, REAfinity™

Certificates

Please follow this
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to search for Certificates of Analysis (CoA) by lot number.

References for CD41/CD61 Antibody, anti-human, REAfinity™

Publications

  1. Naik, U. P. et al. (1997) Identification of a novel calcium-binding protein that interacts with the integrin alphaIIb cytoplasmic domain. J. Biol. Chem. 272(8): 4651-4654
  2. Podolnikova, N. P. et al. (2014) The interaction of integrin αIIbβ3 with fibrin occurs through multiple binding sites in the αIIb β-propeller domain. J. Biol. Chem. 289(4): 2371-2383
  3. Corbel, C. et al. (2005) (alpha)IIb integrin, a novel marker for hemopoietic progenitor cells. Int. J. Dev. Biol. 49(2-3): 279-284

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