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Splenocytes from C56BL/6 mice were stained with REAlease CD54 (ICAM-1)-APC (B) or with the corresponding REAL Control (A). REAlease CD54 (ICAM-1)-APC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD54 (ICAM-1)-APC (D). Cells were analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.