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Mouse PDCs were specifically depleted in BALB/c mice by i.p. injection of 500 µg CD317 (PDCA-1) pure – functional grade. The frequency of CD317 (PDCA-1)+
PDCs was analyzed in spleen 24 hours after injection of the antibody or diluent only (control mice) by staining with CD317 (PDCA-1) antibodies. To exclude blocking of CD317 (PDCA-1) staining by thein vivo
applied CD317 (PDCA-1) antibody, cells were additionally stained with Anti-Ly-6C and CD45R (B220) antibodies. Note that the frequency of PDCs decreases in mice which received the CD317 (PDCA-1) antibody (A) to about one fifth compared to control mice (B), independent of whether it is analyzed based on CD317 (PDCA-1) staining or on Anti-Ly-6C and CD45R (B220) staining. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
A) Anti-mPDCA-1 antibody
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