CD133/1 (AC133) antibodies, human

CD133/1 (AC133) antibodies, human

Clone: AC133 | Dilution: 1:50
CD133, formerly known as AC133, recognizes epitope 1 of the CD133 antigen. It is a marker that is frequently found on multipotent progenitor cells, including immature hematopoietic stem and progenitor cells. In the hematopoietic system, CD133 is expressed on a small portion of CD34
cells as well as on a subset of CD34
bright
stem and progenitor cells in human fetal liver, bone marrow, cord blood, and peripheral blood. CD133 has also been found to be expressed on circulating endothelial progenitor cells, fetal neural stem cells, other tissue-specific stem cells, such as renal, prostate, and corneal stem cells, cancer stem cells from tumor tissues, as well as ES and iPS cell–derived cells.

Alternative names

PROM1, AC133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4

Technical specifications

  • Antigen: CD133/1 (AC133)
  • Clone: AC133
  • Isotype: mouse IgG1κ
  • Isotype control: Mouse IgG1 – isotype control antibodies
  • Alternative names of antigen: PROM1, AC133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4
  • Entrez Gene ID: 8842
  • Molecular mass of antigen [kDa]: 95
  • Distribution of antigen: brain, endothelial cells, epithelial cells, heart, hematopoietic stem cells, kidney, liver, lung, pancreas, placenta, ES and iPS cells, red blood cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, APC, PE-Vio615, PE-Vio770, VioBright 515, Biotin
  • Selected references

    1. Fredebohm, J. et al. (2012) Establishment and characterization of a highly tumourigenic and cancer stem cell enriched pancreatic cancer cell line as a well defined model system. PLoS One 7(11): e48503
    2. Balasubramanian, P. et al. (2013)
      AQP9 expression in glioblastoma multiforme tumors is limited to a small population of astrocytic cells and CD15
      +
      /CalB
      +
      leukocytes.
      PLoS One 8(9): e75764
    3. Metsuyanim, S. et al. (2009) Expression of stem cell markers in the human fetal kidney. PLoS One 4(68): e6709
    4. Qi, Y. et al. (2012) Inhaled NO contributes to lung repair in piglets with acute respiratory distress syndrome via increasing circulating endothelial progenitor cells. PLoS One 7(3): e33859
    5. Ye, X. et al. (2013) The effect of Heparin-VEGF multilayer on the biocompatibility of decellularized aortic valve with platelet and endothelial progenitor cells. PLoS One 8(1): e54622
    6. Hu, Y. et al. (2013) Tumor-specific chromosome mis-segregation controls cancer plasticity by maintaining tumor heterogeneity. PLoS One 8(11): e80898
    7. de Wynter, E. A. et al. (1998)
      CD34
      +
      AC133
      +
      cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors.
      Stem Cells 16: 387-396
    8. Alvarez, D. F. et al. (2008) Lung microvascular endothelium is enriched with progenitor cells that exhibit vasculogenic capacity. Am. J. Physiol. Lung Cell Mol. Physiol. 294(3): L419-L430
    9. Rheinbay, E. et al. (2013) An aberrant transcription factor network essential for Wnt signaling and stem cell maintenance in glioblastoma. Cell Rep 3(5): 1567-1579
    10. Behbod, F. et al. (2009)
      An intraductal human-in-mouse transplantation model mimics the subtypes of ductal carcinoma
      in situ
      .
      Breast Cancer Res. 11(5): R66
    11. Steinmetz, N. F. et al. (2011) Two domains of vimentin are expressed on the surface of lymph node, bone and brain metastatic prostate cancer lines along with the putative stem cell marker proteins CD44 and CD133. Cancers (Basel) 3(3): 2870-2885
    12. Liu, H. et al. (2013) Single-cell clones of liver cancer stem cells have the potential of differentiating into different types of tumor cells. Cell Death Dis 4: e857
    13. Czyz, M. et al. (2013) Parthenolide reduces the frequency of ABCB5-positive cells and clonogenic capacity of melanoma cells from anchorage independent melanospheres. Cancer Biol. Ther. 14(2): 135-145
    14. Tsang, H. et al. (2013) Role of asymmetric methylarginine and connexin 43 in the regulation of pulmonary endothelial function. Pulm Circ 3(3): 675-691
    15. Sun, Y. et al. (2014) Slug overexpression induces stemness and promotes hepatocellular carcinoma cell invasion and metastasis. Oncol Lett 7(6): 1936-1940
    16. Schiffer, D. et al. (2014) Stem cell niches in glioblastoma: a neuropathological view. Biomed Res Int 2014: 725921
    17. Giebel, B. et al. (2004) Segregation of lipid raft markers including CD133 in polarized human hematopoietic stem and progenitor cells. Blood 104(8): 2332-2338
    18. Ingram, D. A. et al. (2004) Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood. Blood 104(9): 2752-2760
    19. Miraglia, S. et al. (1997) A novel five-transmembrane hematopoietic stem cell antigen: isolation, characterization, and molecular cloning. Blood 90(12): 5013-5021
    20. Wagner, W. et al. (2004) Molecular evidence for stem cell function of the slow-dividing fraction among human hematopoietic progenitor cells by genome-wide analysis. Blood 104(3): 675-686
    21. Yin, A. H. et al. (1997) AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 90: 5002-5012
    22. Gallacher, L. et al. (2000)
      Isolation and characterization of human CD34
      Lin
      and CD34
      +
      Lin
      hematopoietic stem cells using cell surface markers AC133 and CD7.
      Blood 95(ARVO Annual Meeting Abstract): 2813-2820
    23. Gehling, U. M. et al. (2000)
      In vitro
      differentiation of endothelial cells from AC133-positive progenitor cells.
      Blood 95: 3106-3112
    24. Peichev, M. et al. (2000)
      Expression of VEGFR-2 and AC133 by circulating human CD34
      +
      cells identifies a population of functional endothelial precursors.
      Blood 95: 952-958
    25. Cummings, B. J. et al. (2005) Human neural stem cells differentiate and promote locometer recovery in spinal cord-injured mice. Proc. Natl. Acad. Sci. U.S.A. 102: 14069-14074
    26. Galic, Z. et al. (2006) T lineage differentiation from human embryonic stem cells. Proc. Natl. Acad. Sci. U.S.A. 103: 11742-11747
    27. Uchida, N. et al. (2000) Direct isolation of human central nervous system stem cells. Proc. Natl. Acad. Sci. U.S.A. 97: 14720-14725
    28. Weigmann, A. et al. (1997) Prominin, a novel microvilli-specific polytopic membrane proteine of the apical surface of epithelial cells, is targeted to plasmalemmal protrusions of non-epithelial cells. Proc. Natl. Acad. Sci. U.S.A. 94: 12425-12430
    29. Estes, M. L. et al. (2010) Application of polychromatic flow cytometry to identify novel subsets of circulating cells with angiogenic potential. Cytometry A 77(9): 831-839
    30. Park, T. S. et al. (2013) Efficient and simultaneous generation of hematopoietic and vascular progenitors from human induced pluripotent stem cells. Cytometry A 83(1): 114-126
    31. Bussolati, B. et al. (2005) Isolation of renal progenitor cells from adult human kidney. Am. J. Pathol. 166: 545-555
    32. Bühring, H. J. et al. (1999) Expression of novel surface antigens on early hematopoietic cells. Ann. N. Y. Acad. Sci. 872: 25-39
    33. Immervoll, H. et al. (2008) Expression of the "stem cell marker" CD133 in pancreas and pancreatic ductal adenocarcinomas. BMC Cancer 8: 48
    34. Wong, C. K. E. et al. (2012) Levels of a subpopulation of platelets, but not circulating endothelial cells, predict early treatment failure in prostate cancer patients after prostatectomy. Br. J. Cancer 107(9): 1564-1573
    35. Richardson, G. et al. (2004) CD133, a novel marker for human prostatic epithelial stem cells. J. Cell. Sci. 117: 3539-3545
    36. Walton, R. M. et al. (2013)
      Postnatal neural precursor cell regions in the rostral subventricular zone, hippocampal subgranular zone and cerebellum of the dog (
      Canis lupus familiaris
      ).
      Histochem. Cell Biol. 139(3): 415-429
    37. Fusi, A. et al. (2011) Expression of the stem cell markers nestin and CD133 on circulating melanoma cells. J. Invest. Dermatol. 131(2): 487-494
    38. Thill, M. et al. (2004)
      Identification of a population of CD133
      +
      precursor cells in the stroma of human cornea.
      Invest. Ophthalmol. Vis. Sci. 45: 3519
    39. Fonseca, A. V. et al. (2010) Polarization and migration of hematopoietic stem and progenitor cells rely on the J. Biol. Chem. 285(41): 31661-31671
    40. Mak, A. B. et al. (2011) CD133 protein N-glycosylation processing contributes to cell surface recognition of the primitive cell marker AC133 epitope. J. Biol. Chem. 286(47): 41046-41056
    41. Maxwell, S. A. et al. (2009) 14-3-3zeta J. Biol. Chem. 284(33): 22379-22389
    42. Piechaczek, C. (2001) CD133. J. Biol. Regul. Homeost. Agents 15: 101-102
    43. Margolis, D. J. et al. (2009) Phase I study of H5.020CMV.PDGF-beta to treat venous leg ulcer disease. Mol. Ther. 17(10): 1822-1829
    44. Duda, D. G. et al. (2007) A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood. Nat. Protoc. 2(4): 805-810
    45. Clark, P. A. et al. (2012) Activation of multiple ERBB family receptors mediates glioblastoma cancer stem-like cell resistance to EGFR-targeted inhibition. Neoplasia 14(5): 420-428
    46. Lin, L. et al. (2009) The STAT3 inhibitor NSC 74859 is effective in hepatocellular cancers with Oncogene 28(7): 961-972
    47. Ferro, F. et al. (2012) Dental pulp stem cells differentiation reveals new insights in Oct4A dynamics. PLoS One 7(7): e41774
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